Pharmacological activity of chaga mushroom on extraction conditions and immunostimulating polysaccharide

Gil Hun Baek, Heon Sang Jeong, Hoon Kim, Taek Joon Yoon, Hyung Joo Suh, Kwang Won Yu

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


To investigate the pharmacological activity of chaga mushroom (Inonotus obliquus) on extraction conditions, chaga was extracted using water (reflux at 50°C, decoction over 90°C, pressure at 121°C) or ethanol (reflux at 50, 70, or 90°C). When water extract was further fractionated into crude polysaccharide (IO-CP), yields of IO-CP (4.8~16.8%) were higher than those of ethanolic extracts (IO-E, 1.9~2.7%) at increased temperature. For antioxidant activity, crude polysaccharide (IO-CP-121) obtained by pressurized extraction showed the highest polyphenolic and flavonoid contents (35.10 mg TAE/g and 18.48 mg QE/g, respectively) as well as DPPH and ABTS free radical scavenging activities (26.08 and 27.99 mg AEAC/100 mg, respectively). Meanwhile, IO-CP-D (decoction) and IO-CP-50 (reflux) had more potent mitogenic effects (2.10- and 1.95-fold of saline control at 100 μg/mL) as well as intestinal immune system modulating activities (6.30- and 5.74-fold) compared to IO-CP-121, whereas ethanolic extracts showed no activity. Although no IO-CP showed cytotoxicity against RAW 264.7 cells at 0.1 mg/mL, IO-CP-121 significantly inhibited TNF-α and NO production as pro-inflammatory factors in LPS-stimulated RAW 264.7 cells (29.2 and 63.5%, respectively). Ethanolic extracts also showed no cytotoxicity at 0.1 mg/mL, whereas inhibition of TNF-α and NO production was significantly low compared to that of IO-CP-121. In addition, active IO-CP-D was further fractionated into an unadsorbed (IO-CP-I) and seven adsorbed fractions (IO-CP-II~VIII) by DEAE-Sepharose CL-6B column chromatography in order to isolate immunostimulating polysaccharide. IO-CP-II showed the most potent mitogenic effect and macrophage stimulating activity (4.51- and 1.64-fold, respectively). IO-CP-II mainly contained neutral sugars (61.86%) in addition to a small amount of uronic acid (2.96%), and component sugar analysis showed that IO-CP-II consisted mainly of Glc, Gal, and Man (molar ratio of 1.00:0.55:0.31). Therefore, extraction conditions affect the physiological activity of chaga, and immunostimulating polysaccharide fractionated from chaga by decoction is composed mainly of neutral sugars.

Original languageEnglish
Pages (from-to)1378-1387
Number of pages10
JournalJournal of the Korean Society of Food Science and Nutrition
Issue number10
Publication statusPublished - 2012


  • Chaga mushroom
  • Crude polysaccharide
  • Extraction condition
  • Pharmacological activity
  • Solvent extract

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Food Science


Dive into the research topics of 'Pharmacological activity of chaga mushroom on extraction conditions and immunostimulating polysaccharide'. Together they form a unique fingerprint.

Cite this