Phc2 controls hematopoietic stem and progenitor cell mobilization from bone marrow by repressing Vcam1 expression

Joonbeom Bae, Sang Pil Choi, Kyoichi Isono, Ji Yoon Lee, Si Won Park, Chang Yong Choi, Jihye Han, Sang Hoon Kim, Han Hyoung Lee, Kyungmin Park, Hyun Yong Jin, Suk Jun Lee, Chung Gyu Park, Haruhiko Koseki, Young Sik Lee, Taehoon Chun

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The timely mobilization of hematopoietic stem and progenitor cells (HSPCs) is essential for maintaining hematopoietic and tissue leukocyte homeostasis. Understanding how HSPCs migrate between bone marrow (BM) and peripheral tissues is of great significance in the clinical setting, where therapeutic strategies for modulating their migration capacity determine the clinical outcome. Here, we identify an epigenetic regulator, Phc2, as a critical modulator of HSPC trafficking. The genetic ablation of Phc2 in mice causes a severe defect in HSPC mobilization through the derepression of Vcam1 in bone marrow stromal cells (BMSCs), ultimately leading to a systemic immunodeficiency. Moreover, the pharmacological inhibition of VCAM-1 in Phc2-deficient mice reverses the symptoms. We further determine that Phc2-dependent Vcam1 repression in BMSCs is mediated by the epigenetic regulation of H3K27me3 and H2AK119ub. Together, our data demonstrate a cell-extrinsic role for Phc2 in controlling the mobilization of HSPCs by finely tuning their bone marrow niche.

Original languageEnglish
Article number3496
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

Bibliographical note

Funding Information:
We thank Amgen Inc. (Thousand Oaks, CA, USA) for providing G-CSF. We also thank Eun-Kyeong Jo (Chungnam National University School of Medicine), Kyung-Mi Lee (Korea University College of Medicine), Seungkwon You (Korea University School of Life Sciences and Biotechnology), and Seok-Ho Hong (Kangwon National University College of Medicine) for their critical reading of our manuscript or helpful suggestions. This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT (2017M3A9C8060392), and an intramural research program funded by Korea University grant (2016).

Publisher Copyright:
© 2019, The Author(s).

ASJC Scopus subject areas

  • General Physics and Astronomy
  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology

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