Phenanthroquinolizidine alkaloids from the roots of Boehmeria pannosa potently inhibit hypoxia-inducible factor-1 in AGS human gastric cancer cells

Xing Fu Cai; Xuejun Jin; Dongho Lee; Young Taek Yang; Kyeong Lee; Young Soo Hong; Jeong Hyung Lee; Jung Joon Lee

doi:10.1021/np060081y

Phenanthroquinolizidine alkaloids from the roots of Boehmeria pannosa potently inhibit hypoxia-inducible factor-1 in AGS human gastric cancer cells

Xing Fu Cai, Xuejun Jin, Dongho Lee, Young Taek Yang, Kyeong Lee, Young Soo Hong, Jeong Hyung Lee, Jung Joon Lee

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

A bioassay-guided phytochemical investigation on the methanol extract of Boehmeria pannosa, using a HIF-1-mediated reporter gene assay, led to the isolation of two phenanthroquinolizidine alkaloids, (-)-cryptopleurine (1) and (-)-(15R)-hydroxycryptopleurine (2). The structure of the new compound 2 was determined by spectroscopic methods. Compounds 1 and 2 potently inhibited the hypoxia-induced expression of a reporter gene under the control of a hypoxia response element (HRE) with IC₅₀ values of 8.7 and 48.1 nM, respectively. Furthermore, 1 and 2 suppressed the accumulation of HEF-1α protein in a dose-dependent manner, but not the HIF-1β protein and inhibited expression of vascular endothelial growth factor (VEGF) by hypoxia.

Original language	English
Pages (from-to)	1095-1097
Number of pages	3
Journal	Journal of Natural Products
Volume	69
Issue number	7
DOIs	https://doi.org/10.1021/np060081y
Publication status	Published - 2006 Jul
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/np060081y

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title = "Phenanthroquinolizidine alkaloids from the roots of Boehmeria pannosa potently inhibit hypoxia-inducible factor-1 in AGS human gastric cancer cells",

abstract = "A bioassay-guided phytochemical investigation on the methanol extract of Boehmeria pannosa, using a HIF-1-mediated reporter gene assay, led to the isolation of two phenanthroquinolizidine alkaloids, (-)-cryptopleurine (1) and (-)-(15R)-hydroxycryptopleurine (2). The structure of the new compound 2 was determined by spectroscopic methods. Compounds 1 and 2 potently inhibited the hypoxia-induced expression of a reporter gene under the control of a hypoxia response element (HRE) with IC50 values of 8.7 and 48.1 nM, respectively. Furthermore, 1 and 2 suppressed the accumulation of HEF-1α protein in a dose-dependent manner, but not the HIF-1β protein and inhibited expression of vascular endothelial growth factor (VEGF) by hypoxia.",

author = "Cai, {Xing Fu} and Xuejun Jin and Dongho Lee and Yang, {Young Taek} and Kyeong Lee and Hong, {Young Soo} and Lee, {Jeong Hyung} and Lee, {Jung Joon}",

year = "2006",

month = jul,

doi = "10.1021/np060081y",

language = "English",

volume = "69",

pages = "1095--1097",

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publisher = "American Chemical Society",

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T1 - Phenanthroquinolizidine alkaloids from the roots of Boehmeria pannosa potently inhibit hypoxia-inducible factor-1 in AGS human gastric cancer cells

AU - Cai, Xing Fu

AU - Jin, Xuejun

AU - Lee, Dongho

AU - Yang, Young Taek

AU - Lee, Kyeong

AU - Hong, Young Soo

AU - Lee, Jeong Hyung

AU - Lee, Jung Joon

PY - 2006/7

Y1 - 2006/7

N2 - A bioassay-guided phytochemical investigation on the methanol extract of Boehmeria pannosa, using a HIF-1-mediated reporter gene assay, led to the isolation of two phenanthroquinolizidine alkaloids, (-)-cryptopleurine (1) and (-)-(15R)-hydroxycryptopleurine (2). The structure of the new compound 2 was determined by spectroscopic methods. Compounds 1 and 2 potently inhibited the hypoxia-induced expression of a reporter gene under the control of a hypoxia response element (HRE) with IC50 values of 8.7 and 48.1 nM, respectively. Furthermore, 1 and 2 suppressed the accumulation of HEF-1α protein in a dose-dependent manner, but not the HIF-1β protein and inhibited expression of vascular endothelial growth factor (VEGF) by hypoxia.

AB - A bioassay-guided phytochemical investigation on the methanol extract of Boehmeria pannosa, using a HIF-1-mediated reporter gene assay, led to the isolation of two phenanthroquinolizidine alkaloids, (-)-cryptopleurine (1) and (-)-(15R)-hydroxycryptopleurine (2). The structure of the new compound 2 was determined by spectroscopic methods. Compounds 1 and 2 potently inhibited the hypoxia-induced expression of a reporter gene under the control of a hypoxia response element (HRE) with IC50 values of 8.7 and 48.1 nM, respectively. Furthermore, 1 and 2 suppressed the accumulation of HEF-1α protein in a dose-dependent manner, but not the HIF-1β protein and inhibited expression of vascular endothelial growth factor (VEGF) by hypoxia.

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Phenanthroquinolizidine alkaloids from the roots of Boehmeria pannosa potently inhibit hypoxia-inducible factor-1 in AGS human gastric cancer cells

Abstract

ASJC Scopus subject areas

UN SDGs

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