Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity

Yun Hyeok Choi; Seong Su Hong; Yu Su Shin; Bang Yeon Hwang; So Young Park; Dongho Lee

doi:10.1007/s12272-010-1014-7

Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity

Yun Hyeok Choi, Seong Su Hong, Yu Su Shin, Bang Yeon Hwang, So Young Park, Dongho Lee

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Bioassay-guided fractionation of the EtOAc-soluble extract of Pueraria lobata based on the inhibition of Aβ-induced toxicity in PC12 cells resulted in the isolation of four known active compounds, genistein (8), biochanin A (9), sissotrin (10), and puerol B (11). Of these, genistein (8) and biochanin A (9) exhibited potent neuroprotective effects with ED₅₀ values of 33.7 and 27.8 μM, respectively. In addition, a new coumestan, 2-(α,α-dimethylallyl)coumestrol (1) was isolated and characterized, but proved to be inactive, as were additional seven known compounds. The structure of new compound 1 was determined using spectroscopic techniques.

Original language	English
Pages (from-to)	1651-1654
Number of pages	4
Journal	Archives of pharmacal research
Volume	33
Issue number	10
DOIs	https://doi.org/10.1007/s12272-010-1014-7
Publication status	Published - 2010 Oct

Keywords

2-(α,α-dimethylallyl)coumestrol
Beta-amyloid
Fabaceae
Isoflavone
Pueraria lobata

ASJC Scopus subject areas

Molecular Medicine
Drug Discovery
Organic Chemistry

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10.1007/s12272-010-1014-7

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title = "Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity",

abstract = "Bioassay-guided fractionation of the EtOAc-soluble extract of Pueraria lobata based on the inhibition of Aβ-induced toxicity in PC12 cells resulted in the isolation of four known active compounds, genistein (8), biochanin A (9), sissotrin (10), and puerol B (11). Of these, genistein (8) and biochanin A (9) exhibited potent neuroprotective effects with ED50 values of 33.7 and 27.8 μM, respectively. In addition, a new coumestan, 2-(α,α-dimethylallyl)coumestrol (1) was isolated and characterized, but proved to be inactive, as were additional seven known compounds. The structure of new compound 1 was determined using spectroscopic techniques.",

keywords = "2-(α,α-dimethylallyl)coumestrol, Beta-amyloid, Fabaceae, Isoflavone, Pueraria lobata",

author = "Choi, {Yun Hyeok} and Hong, {Seong Su} and Shin, {Yu Su} and Hwang, {Bang Yeon} and Park, {So Young} and Dongho Lee",

note = "Funding Information: This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. We thank Cooperative Center for Analytical Instruments at Korea University for providing some of the equipment used in this study.",

year = "2010",

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AU - Choi, Yun Hyeok

AU - Hong, Seong Su

AU - Shin, Yu Su

AU - Hwang, Bang Yeon

AU - Park, So Young

AU - Lee, Dongho

N1 - Funding Information: This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. We thank Cooperative Center for Analytical Instruments at Korea University for providing some of the equipment used in this study.

PY - 2010/10

Y1 - 2010/10

N2 - Bioassay-guided fractionation of the EtOAc-soluble extract of Pueraria lobata based on the inhibition of Aβ-induced toxicity in PC12 cells resulted in the isolation of four known active compounds, genistein (8), biochanin A (9), sissotrin (10), and puerol B (11). Of these, genistein (8) and biochanin A (9) exhibited potent neuroprotective effects with ED50 values of 33.7 and 27.8 μM, respectively. In addition, a new coumestan, 2-(α,α-dimethylallyl)coumestrol (1) was isolated and characterized, but proved to be inactive, as were additional seven known compounds. The structure of new compound 1 was determined using spectroscopic techniques.

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KW - 2-(α,α-dimethylallyl)coumestrol

KW - Beta-amyloid

KW - Fabaceae

KW - Isoflavone

KW - Pueraria lobata

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Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity

Abstract

Keywords

ASJC Scopus subject areas

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