Phenylobacterium parvum sp. Nov., isolated from lake water

Chaeyun Baek; Su Kyoung Shin; Hana Yi

doi:10.1099/ijsem.0.003284

Phenylobacterium parvum sp. Nov., isolated from lake water

Chaeyun Baek, Su Kyoung Shin, Hana Yi

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7 Citations (Scopus)

Abstract

A Gram-stain-negative, rod-shaped and aerobic bacterium, designated HYN0004^T, was isolated from lake water. The strain grew at 15–35^°C and pH 7.0–9.0 on R2A. The isoprenoid quinone was Q10 and major polar lipids were phosphatidylglycerol and one unidentified glycolipid. The genome was 2.83 Mb with a DNA G+C content of 69.9 mol%. 16S rRNA gene sequence analyses revealed that HYN0004^T represented a member of the genus Phenylobacterium and shared sequence similarities with Phenylobacterium conjunctum (97.8 %), Phenylobacterium koreense (97.5 %), Phenylobacterium aquaticum (97.2 %), and Phenylobacteriumheamatophilum (97.0 %). In addition to the low sequence similarities, the phylogenetic tree shapes indicated that HYN0004^Trepresents an independent species of this genus. The genomic and phenotypic properties, including small genome size, inability to carry out numerous enzymatic reactions and high ratio of C_{18: 1}ω6c and/or C_{18: 1}ω7c in fatty acids, verified the differentiation between HYN0004^T and related species. Thus, we propose a novel species of the genus Phenylobacterium, named as Phenylobacterium parvum sp. nov. The type strain is HYN0004^T (=KACC 19185^T=NBRC 112736^T).

Original language	English
Article number	003284
Pages (from-to)	1169-1172
Number of pages	4
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	69
Issue number	4
DOIs	https://doi.org/10.1099/ijsem.0.003284
Publication status	Published - 2019 Apr

Bibliographical note

Funding Information:
This study was supported by the research grant ‘Survey of Freshwater Organisms and Specimen Collection (Prokaryotes)’ from the Nakdong-gang National Institute of Biological Resources of the Ministry of Environment in Korea, by the Basic Science Research Programs through the National Research Foundation of Korea (NRF), funded by the Korean Ministry of Education (2015R1D1A1A01057527), and by the Strategic Initiative for Microbiomes in Agriculture and Food, funded by the Ministry of Agriculture, Food and Rural Affairs (918010–4).

Publisher Copyright:
© 2019 IUMS.

Keywords

Lake water
Nakdong River
Phenylobacterium

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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10.1099/ijsem.0.003284

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title = "Phenylobacterium parvum sp. Nov., isolated from lake water",

abstract = "A Gram-stain-negative, rod-shaped and aerobic bacterium, designated HYN0004T, was isolated from lake water. The strain grew at 15–35°C and pH 7.0–9.0 on R2A. The isoprenoid quinone was Q10 and major polar lipids were phosphatidylglycerol and one unidentified glycolipid. The genome was 2.83 Mb with a DNA G+C content of 69.9 mol%. 16S rRNA gene sequence analyses revealed that HYN0004T represented a member of the genus Phenylobacterium and shared sequence similarities with Phenylobacterium conjunctum (97.8 %), Phenylobacterium koreense (97.5 %), Phenylobacterium aquaticum (97.2 %), and Phenylobacteriumheamatophilum (97.0 %). In addition to the low sequence similarities, the phylogenetic tree shapes indicated that HYN0004Trepresents an independent species of this genus. The genomic and phenotypic properties, including small genome size, inability to carry out numerous enzymatic reactions and high ratio of C18: 1ω6c and/or C18: 1ω7c in fatty acids, verified the differentiation between HYN0004T and related species. Thus, we propose a novel species of the genus Phenylobacterium, named as Phenylobacterium parvum sp. nov. The type strain is HYN0004T (=KACC 19185T=NBRC 112736T).",

keywords = "Lake water, Nakdong River, Phenylobacterium",

author = "Chaeyun Baek and Shin, {Su Kyoung} and Hana Yi",

note = "Funding Information: This study was supported by the research grant {\textquoteleft}Survey of Freshwater Organisms and Specimen Collection (Prokaryotes){\textquoteright} from the Nakdong-gang National Institute of Biological Resources of the Ministry of Environment in Korea, by the Basic Science Research Programs through the National Research Foundation of Korea (NRF), funded by the Korean Ministry of Education (2015R1D1A1A01057527), and by the Strategic Initiative for Microbiomes in Agriculture and Food, funded by the Ministry of Agriculture, Food and Rural Affairs (918010–4). Publisher Copyright: {\textcopyright} 2019 IUMS.",

year = "2019",

month = apr,

doi = "10.1099/ijsem.0.003284",

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volume = "69",

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AU - Baek, Chaeyun

AU - Shin, Su Kyoung

AU - Yi, Hana

N1 - Funding Information: This study was supported by the research grant ‘Survey of Freshwater Organisms and Specimen Collection (Prokaryotes)’ from the Nakdong-gang National Institute of Biological Resources of the Ministry of Environment in Korea, by the Basic Science Research Programs through the National Research Foundation of Korea (NRF), funded by the Korean Ministry of Education (2015R1D1A1A01057527), and by the Strategic Initiative for Microbiomes in Agriculture and Food, funded by the Ministry of Agriculture, Food and Rural Affairs (918010–4). Publisher Copyright: © 2019 IUMS.

PY - 2019/4

Y1 - 2019/4

N2 - A Gram-stain-negative, rod-shaped and aerobic bacterium, designated HYN0004T, was isolated from lake water. The strain grew at 15–35°C and pH 7.0–9.0 on R2A. The isoprenoid quinone was Q10 and major polar lipids were phosphatidylglycerol and one unidentified glycolipid. The genome was 2.83 Mb with a DNA G+C content of 69.9 mol%. 16S rRNA gene sequence analyses revealed that HYN0004T represented a member of the genus Phenylobacterium and shared sequence similarities with Phenylobacterium conjunctum (97.8 %), Phenylobacterium koreense (97.5 %), Phenylobacterium aquaticum (97.2 %), and Phenylobacteriumheamatophilum (97.0 %). In addition to the low sequence similarities, the phylogenetic tree shapes indicated that HYN0004Trepresents an independent species of this genus. The genomic and phenotypic properties, including small genome size, inability to carry out numerous enzymatic reactions and high ratio of C18: 1ω6c and/or C18: 1ω7c in fatty acids, verified the differentiation between HYN0004T and related species. Thus, we propose a novel species of the genus Phenylobacterium, named as Phenylobacterium parvum sp. nov. The type strain is HYN0004T (=KACC 19185T=NBRC 112736T).

AB - A Gram-stain-negative, rod-shaped and aerobic bacterium, designated HYN0004T, was isolated from lake water. The strain grew at 15–35°C and pH 7.0–9.0 on R2A. The isoprenoid quinone was Q10 and major polar lipids were phosphatidylglycerol and one unidentified glycolipid. The genome was 2.83 Mb with a DNA G+C content of 69.9 mol%. 16S rRNA gene sequence analyses revealed that HYN0004T represented a member of the genus Phenylobacterium and shared sequence similarities with Phenylobacterium conjunctum (97.8 %), Phenylobacterium koreense (97.5 %), Phenylobacterium aquaticum (97.2 %), and Phenylobacteriumheamatophilum (97.0 %). In addition to the low sequence similarities, the phylogenetic tree shapes indicated that HYN0004Trepresents an independent species of this genus. The genomic and phenotypic properties, including small genome size, inability to carry out numerous enzymatic reactions and high ratio of C18: 1ω6c and/or C18: 1ω7c in fatty acids, verified the differentiation between HYN0004T and related species. Thus, we propose a novel species of the genus Phenylobacterium, named as Phenylobacterium parvum sp. nov. The type strain is HYN0004T (=KACC 19185T=NBRC 112736T).

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JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 4

M1 - 003284

ER -

Phenylobacterium parvum sp. Nov., isolated from lake water

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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