Phospholipase A1-catalyzed synthesis of phospholipids enriched in n - 3 polyunsaturated fatty acid residues

In Hwan Kim; Hugo S. Garcia; Charles Graham Hill

doi:10.1016/j.enzmictec.2006.08.018

Phospholipase A₁-catalyzed synthesis of phospholipids enriched in n - 3 polyunsaturated fatty acid residues

In Hwan Kim, Hugo S. Garcia, Charles Graham Hill

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

Phosphatidylcholine (PC) was successfully modified by phospholipase A₁-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A₁ from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6 h of reaction at 55 °C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PUFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC.

Original language	English
Pages (from-to)	1130-1135
Number of pages	6
Journal	Enzyme and Microbial Technology
Volume	40
Issue number	5
DOIs	https://doi.org/10.1016/j.enzmictec.2006.08.018
Publication status	Published - 2007 Apr 3

Bibliographical note

Funding Information:
This work was supported by the University of Wisconsin Sea Grant Institute under grants from the National Sea Grant College Program, National Oceanic and Atmospheric Administration, US Department of Commerce, and from the State of Wisconsin (Federal grant number NA 46 RG 0481, project number R/AQ-34). Additional support was provided by National Science Foundation Grant BES-077524. We also thank Mr. Iswandi Jarto for his contributions to the experimental work.

Keywords

Acidolysis
Fish oil
Phosphatidylcholine
Phospholipase A
n - 3 polyunsaturated fatty acid (PUFA)

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

Access to Document

10.1016/j.enzmictec.2006.08.018

Cite this

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title = "Phospholipase A1-catalyzed synthesis of phospholipids enriched in n - 3 polyunsaturated fatty acid residues",

abstract = "Phosphatidylcholine (PC) was successfully modified by phospholipase A1-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A1 from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6 h of reaction at 55 °C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PUFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC.",

keywords = "Acidolysis, Fish oil, Phosphatidylcholine, Phospholipase A, n - 3 polyunsaturated fatty acid (PUFA)",

author = "Kim, {In Hwan} and Garcia, {Hugo S.} and Hill, {Charles Graham}",

note = "Funding Information: This work was supported by the University of Wisconsin Sea Grant Institute under grants from the National Sea Grant College Program, National Oceanic and Atmospheric Administration, US Department of Commerce, and from the State of Wisconsin (Federal grant number NA 46 RG 0481, project number R/AQ-34). Additional support was provided by National Science Foundation Grant BES-077524. We also thank Mr. Iswandi Jarto for his contributions to the experimental work.",

year = "2007",

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doi = "10.1016/j.enzmictec.2006.08.018",

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T1 - Phospholipase A1-catalyzed synthesis of phospholipids enriched in n - 3 polyunsaturated fatty acid residues

AU - Kim, In Hwan

AU - Garcia, Hugo S.

AU - Hill, Charles Graham

N1 - Funding Information: This work was supported by the University of Wisconsin Sea Grant Institute under grants from the National Sea Grant College Program, National Oceanic and Atmospheric Administration, US Department of Commerce, and from the State of Wisconsin (Federal grant number NA 46 RG 0481, project number R/AQ-34). Additional support was provided by National Science Foundation Grant BES-077524. We also thank Mr. Iswandi Jarto for his contributions to the experimental work.

PY - 2007/4/3

Y1 - 2007/4/3

N2 - Phosphatidylcholine (PC) was successfully modified by phospholipase A1-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A1 from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6 h of reaction at 55 °C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PUFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC.

AB - Phosphatidylcholine (PC) was successfully modified by phospholipase A1-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A1 from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6 h of reaction at 55 °C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PUFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC.

KW - Acidolysis

KW - Fish oil

KW - Phosphatidylcholine

KW - Phospholipase A

KW - n - 3 polyunsaturated fatty acid (PUFA)

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U2 - 10.1016/j.enzmictec.2006.08.018

DO - 10.1016/j.enzmictec.2006.08.018

M3 - Article

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SN - 0141-0229

VL - 40

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EP - 1135

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

IS - 5

ER -