Photo-affinity labeling (PAL) in chemical proteomics: A handy tool to investigate protein-protein interactions (PPIs)

Dhiraj P. Murale; Seong Cheol Hong; Md Mamunul Haque; Jun Seok Lee

doi:10.1186/s12953-017-0123-3

Photo-affinity labeling (PAL) in chemical proteomics: A handy tool to investigate protein-protein interactions (PPIs)

Dhiraj P. Murale, Seong Cheol Hong, Md Mamunul Haque, Jun Seok Lee

Research output: Contribution to journal › Review article › peer-review

131 Citations (Scopus)

Abstract

Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

Original language	English
Article number	14
Journal	Proteome Science
Volume	15
Issue number	1
DOIs	https://doi.org/10.1186/s12953-017-0123-3
Publication status	Published - 2017 Jun 24
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 The Author(s).

Keywords

Aryl azide
Benzophenone
Diazirine
Photo-affinity probe
Protein-protein interaction
Quantitative proteomics

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.1186/s12953-017-0123-3

Cite this

@article{627cf3ff04404946b6a28b383b19e1ad,

title = "Photo-affinity labeling (PAL) in chemical proteomics: A handy tool to investigate protein-protein interactions (PPIs)",

abstract = "Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.",

keywords = "Aryl azide, Benzophenone, Diazirine, Photo-affinity probe, Protein-protein interaction, Quantitative proteomics",

author = "Murale, {Dhiraj P.} and Hong, {Seong Cheol} and Haque, {Md Mamunul} and Lee, {Jun Seok}",

note = "Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

month = jun,

day = "24",

doi = "10.1186/s12953-017-0123-3",

language = "English",

volume = "15",

journal = "Proteome Science",

issn = "1477-5956",

publisher = "BioMed Central",

number = "1",

}

TY - JOUR

T1 - Photo-affinity labeling (PAL) in chemical proteomics

T2 - A handy tool to investigate protein-protein interactions (PPIs)

AU - Murale, Dhiraj P.

AU - Hong, Seong Cheol

AU - Haque, Md Mamunul

AU - Lee, Jun Seok

PY - 2017/6/24

Y1 - 2017/6/24

N2 - Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

AB - Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

KW - Aryl azide

KW - Benzophenone

KW - Diazirine

KW - Photo-affinity probe

KW - Protein-protein interaction

KW - Quantitative proteomics

UR - http://www.scopus.com/inward/record.url?scp=85021275870&partnerID=8YFLogxK

U2 - 10.1186/s12953-017-0123-3

DO - 10.1186/s12953-017-0123-3

M3 - Review article

AN - SCOPUS:85021275870

SN - 1477-5956

VL - 15

JO - Proteome Science

JF - Proteome Science

IS - 1

M1 - 14

ER -

Photo-affinity labeling (PAL) in chemical proteomics: A handy tool to investigate protein-protein interactions (PPIs)

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this