Photo-affinity labeling (PAL) in chemical proteomics: A handy tool to investigate protein-protein interactions (PPIs)

Dhiraj P. Murale; Seong Cheol Hong; Md Mamunul Haque; Jun Seok Lee

doi:10.1186/s12953-017-0123-3

Photo-affinity labeling (PAL) in chemical proteomics: A handy tool to investigate protein-protein interactions (PPIs)

Dhiraj P. Murale, Seong Cheol Hong, Md Mamunul Haque, Jun Seok Lee

Research output: Contribution to journal › Review article › peer-review

97 Citations (Scopus)

Abstract

Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

Original language	English
Article number	14
Journal	Proteome Science
Volume	15
Issue number	1
DOIs	https://doi.org/10.1186/s12953-017-0123-3
Publication status	Published - 2017 Jun 24
Externally published	Yes

Keywords

Aryl azide
Benzophenone
Diazirine
Photo-affinity probe
Protein-protein interaction
Quantitative proteomics

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1186/s12953-017-0123-3

Cite this

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title = "Photo-affinity labeling (PAL) in chemical proteomics: A handy tool to investigate protein-protein interactions (PPIs)",

abstract = "Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.",

keywords = "Aryl azide, Benzophenone, Diazirine, Photo-affinity probe, Protein-protein interaction, Quantitative proteomics",

author = "Murale, {Dhiraj P.} and Hong, {Seong Cheol} and Haque, {Md Mamunul} and Lee, {Jun Seok}",

note = "Funding Information: This work was supported by intramural funding from KIST (2E26632/2E26110, CAP-16-02-KIST) and the Bio & Medical Technology Development Program of the National Research Foundation funded by the Ministry of Science, ICT & Future Planning (NRF-2016M3A9B6902060). Dr. Murale is supported by Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2015H1D3A1036045). Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

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doi = "10.1186/s12953-017-0123-3",

language = "English",

volume = "15",

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AU - Murale, Dhiraj P.

AU - Hong, Seong Cheol

AU - Haque, Md Mamunul

AU - Lee, Jun Seok

N1 - Funding Information: This work was supported by intramural funding from KIST (2E26632/2E26110, CAP-16-02-KIST) and the Bio & Medical Technology Development Program of the National Research Foundation funded by the Ministry of Science, ICT & Future Planning (NRF-2016M3A9B6902060). Dr. Murale is supported by Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2015H1D3A1036045). Publisher Copyright: © 2017 The Author(s).

PY - 2017/6/24

Y1 - 2017/6/24

N2 - Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

AB - Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

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KW - Benzophenone

KW - Diazirine

KW - Photo-affinity probe

KW - Protein-protein interaction

KW - Quantitative proteomics

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DO - 10.1186/s12953-017-0123-3

M3 - Review article

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SN - 1477-5956

VL - 15

JO - Proteome Science

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ER -

Photo-affinity labeling (PAL) in chemical proteomics: A handy tool to investigate protein-protein interactions (PPIs)

Abstract

Keywords

ASJC Scopus subject areas

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