Photodissociation tandem mass spectrometry at 266 nm of an aliphatic peptide derivatized with phenyl isothiocyanate and 4-sulfophenyl isothiocyanate

Yeon Oh Joo, Hee Moon Jeong, Ho Lee Yong, Seok Won Hyung, Sang Won Lee, Soo Kim Myung

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    Photodissociation (PD) tandem mass spectra have been obtained at 266 nm for the protonated molecules of a tryptic peptide, ASHLGLAR, and of its phenyl isothiocyanate and 4-sulfophenyl isothiocyanate derivatives, generated by matrix-assisted laser desorption/ionization. Derivatization with the aromatic chromophores greatly reduced the intensity of the laser required for efficient PD. Major fragment ions observed in the three spectra are quite similar. General features of the PD tandem mass spectra and their potential utility for analytical purposes are discussed.

    Original languageEnglish
    Pages (from-to)1283-1288
    Number of pages6
    JournalRapid Communications in Mass Spectrometry
    Volume19
    Issue number10
    DOIs
    Publication statusPublished - 2005

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Spectroscopy
    • Organic Chemistry

    Fingerprint

    Dive into the research topics of 'Photodissociation tandem mass spectrometry at 266 nm of an aliphatic peptide derivatized with phenyl isothiocyanate and 4-sulfophenyl isothiocyanate'. Together they form a unique fingerprint.

    Cite this