Abstract
Photodissociation (PD) tandem mass spectra have been obtained at 266 nm for the protonated molecules of a tryptic peptide, ASHLGLAR, and of its phenyl isothiocyanate and 4-sulfophenyl isothiocyanate derivatives, generated by matrix-assisted laser desorption/ionization. Derivatization with the aromatic chromophores greatly reduced the intensity of the laser required for efficient PD. Major fragment ions observed in the three spectra are quite similar. General features of the PD tandem mass spectra and their potential utility for analytical purposes are discussed.
Original language | English |
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Pages (from-to) | 1283-1288 |
Number of pages | 6 |
Journal | Rapid Communications in Mass Spectrometry |
Volume | 19 |
Issue number | 10 |
DOIs | |
Publication status | Published - 2005 |
ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
- Organic Chemistry