Photosensitizer-loaded bubble-generating mineralized nanoparticles for ultrasound imaging and photodynamic therapy

Dong Jin Park; Kyung Hyun Min; Hong Jae Lee; Kwangmeyung Kim; Ick Chan Kwon; Seo Young Jeong; Sang Cheon Lee

doi:10.1039/c5tb02338f

Photosensitizer-loaded bubble-generating mineralized nanoparticles for ultrasound imaging and photodynamic therapy

Dong Jin Park, Kyung Hyun Min, Hong Jae Lee, Kwangmeyung Kim, Ick Chan Kwon, Seo Young Jeong, Sang Cheon Lee

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

In this work, we have developed photosensitizer-loaded bubble-generating calcium carbonate (CaCO₃)-mineralized nanoparticles that have potential for ultrasound imaging (US)-guided photodynamic therapy (PDT) of tumors. A photosensitizer, chlorin e6 (Ce6)-loaded CaCO₃-mineralized nanoparticles (Ce6-BMNs), was prepared using an anionic block copolymer-templated in situ mineralization method. Ce6-BMNs were composed of the Ce6-loaded CaCO₃ core and the hydrated poly(ethylene glycol) (PEG) shell. Ce6-BMNs exhibited excellent stability under serum conditions. Ce6-BMNs showed enhanced echogenic US signals at tumoral acid pH by generating carbon dioxide (CO₂) bubbles. Ce6-BMNs effectively inhibited Ce6 release at physiological pH (7.4). At a tumoral acidic pH (6.4), Ce6 release was accelerated with CO₂ bubble generation due to the dissolution of the CaCO₃ mineral core. Upon irradiation of Ce6-BMN-treated MCF-7 breast cancer cells, the cell viability dramatically decreased with increasing Ce6 concentration. The phototoxicity of the Ce6-BMNs was much higher than that of free Ce6. On the basis of tumoral pH-responsive CO₂ bubble-generation and simultaneous Ce6 release at the target tumor site, these CaCO₃ mineralized nanoparticles can be considered as promising theranostic nanoparticles for US imaging-guided PDT in the field of tumor therapy.

Original language	English
Pages (from-to)	1219-1227
Number of pages	9
Journal	Journal of Materials Chemistry B
Volume	4
Issue number	7
DOIs	https://doi.org/10.1039/c5tb02338f
Publication status	Published - 2016 Feb 21
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2012R1A5A2051388) and was supported by a grant of the Korea Health Technology R&D project (HI14C0175) through the KHIDI funded by the Ministry of Health & Welfare, Republic of Korea.

Publisher Copyright:
© The Royal Society of Chemistry 2016.

ASJC Scopus subject areas

Chemistry(all)
Biomedical Engineering
Materials Science(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/c5tb02338f

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@article{3fa52f1036474fb7850407380ccf8317,

title = "Photosensitizer-loaded bubble-generating mineralized nanoparticles for ultrasound imaging and photodynamic therapy",

abstract = "In this work, we have developed photosensitizer-loaded bubble-generating calcium carbonate (CaCO3)-mineralized nanoparticles that have potential for ultrasound imaging (US)-guided photodynamic therapy (PDT) of tumors. A photosensitizer, chlorin e6 (Ce6)-loaded CaCO3-mineralized nanoparticles (Ce6-BMNs), was prepared using an anionic block copolymer-templated in situ mineralization method. Ce6-BMNs were composed of the Ce6-loaded CaCO3 core and the hydrated poly(ethylene glycol) (PEG) shell. Ce6-BMNs exhibited excellent stability under serum conditions. Ce6-BMNs showed enhanced echogenic US signals at tumoral acid pH by generating carbon dioxide (CO2) bubbles. Ce6-BMNs effectively inhibited Ce6 release at physiological pH (7.4). At a tumoral acidic pH (6.4), Ce6 release was accelerated with CO2 bubble generation due to the dissolution of the CaCO3 mineral core. Upon irradiation of Ce6-BMN-treated MCF-7 breast cancer cells, the cell viability dramatically decreased with increasing Ce6 concentration. The phototoxicity of the Ce6-BMNs was much higher than that of free Ce6. On the basis of tumoral pH-responsive CO2 bubble-generation and simultaneous Ce6 release at the target tumor site, these CaCO3 mineralized nanoparticles can be considered as promising theranostic nanoparticles for US imaging-guided PDT in the field of tumor therapy.",

author = "Park, {Dong Jin} and Min, {Kyung Hyun} and Lee, {Hong Jae} and Kwangmeyung Kim and Kwon, {Ick Chan} and Jeong, {Seo Young} and Lee, {Sang Cheon}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2012R1A5A2051388) and was supported by a grant of the Korea Health Technology R&D project (HI14C0175) through the KHIDI funded by the Ministry of Health & Welfare, Republic of Korea. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2016.",

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AU - Park, Dong Jin

AU - Min, Kyung Hyun

AU - Lee, Hong Jae

AU - Kim, Kwangmeyung

AU - Kwon, Ick Chan

AU - Jeong, Seo Young

AU - Lee, Sang Cheon

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2012R1A5A2051388) and was supported by a grant of the Korea Health Technology R&D project (HI14C0175) through the KHIDI funded by the Ministry of Health & Welfare, Republic of Korea. Publisher Copyright: © The Royal Society of Chemistry 2016.

PY - 2016/2/21

Y1 - 2016/2/21

N2 - In this work, we have developed photosensitizer-loaded bubble-generating calcium carbonate (CaCO3)-mineralized nanoparticles that have potential for ultrasound imaging (US)-guided photodynamic therapy (PDT) of tumors. A photosensitizer, chlorin e6 (Ce6)-loaded CaCO3-mineralized nanoparticles (Ce6-BMNs), was prepared using an anionic block copolymer-templated in situ mineralization method. Ce6-BMNs were composed of the Ce6-loaded CaCO3 core and the hydrated poly(ethylene glycol) (PEG) shell. Ce6-BMNs exhibited excellent stability under serum conditions. Ce6-BMNs showed enhanced echogenic US signals at tumoral acid pH by generating carbon dioxide (CO2) bubbles. Ce6-BMNs effectively inhibited Ce6 release at physiological pH (7.4). At a tumoral acidic pH (6.4), Ce6 release was accelerated with CO2 bubble generation due to the dissolution of the CaCO3 mineral core. Upon irradiation of Ce6-BMN-treated MCF-7 breast cancer cells, the cell viability dramatically decreased with increasing Ce6 concentration. The phototoxicity of the Ce6-BMNs was much higher than that of free Ce6. On the basis of tumoral pH-responsive CO2 bubble-generation and simultaneous Ce6 release at the target tumor site, these CaCO3 mineralized nanoparticles can be considered as promising theranostic nanoparticles for US imaging-guided PDT in the field of tumor therapy.

AB - In this work, we have developed photosensitizer-loaded bubble-generating calcium carbonate (CaCO3)-mineralized nanoparticles that have potential for ultrasound imaging (US)-guided photodynamic therapy (PDT) of tumors. A photosensitizer, chlorin e6 (Ce6)-loaded CaCO3-mineralized nanoparticles (Ce6-BMNs), was prepared using an anionic block copolymer-templated in situ mineralization method. Ce6-BMNs were composed of the Ce6-loaded CaCO3 core and the hydrated poly(ethylene glycol) (PEG) shell. Ce6-BMNs exhibited excellent stability under serum conditions. Ce6-BMNs showed enhanced echogenic US signals at tumoral acid pH by generating carbon dioxide (CO2) bubbles. Ce6-BMNs effectively inhibited Ce6 release at physiological pH (7.4). At a tumoral acidic pH (6.4), Ce6 release was accelerated with CO2 bubble generation due to the dissolution of the CaCO3 mineral core. Upon irradiation of Ce6-BMN-treated MCF-7 breast cancer cells, the cell viability dramatically decreased with increasing Ce6 concentration. The phototoxicity of the Ce6-BMNs was much higher than that of free Ce6. On the basis of tumoral pH-responsive CO2 bubble-generation and simultaneous Ce6 release at the target tumor site, these CaCO3 mineralized nanoparticles can be considered as promising theranostic nanoparticles for US imaging-guided PDT in the field of tumor therapy.

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JF - Journal of Materials Chemistry B

IS - 7

ER -

Photosensitizer-loaded bubble-generating mineralized nanoparticles for ultrasound imaging and photodynamic therapy

Abstract

Bibliographical note

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