Photosynthetic CO2 Conversion to Fatty Acid Ethyl Esters (FAEEs) Using Engineered Cyanobacteria

Hyun Jeong Lee; Jaeyeon Choi; Sun Mi Lee; Youngsoon Um; Sang Jun Sim; Yunje Kim; Han Min Woo

doi:10.1021/acs.jafc.7b00002

Photosynthetic CO₂ Conversion to Fatty Acid Ethyl Esters (FAEEs) Using Engineered Cyanobacteria

Hyun Jeong Lee, Jaeyeon Choi, Sun Mi Lee, Youngsoon Um, Sang Jun Sim, Yunje Kim, Han Min Woo

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Metabolic engineering of cyanobacteria has received attention as a sustainable strategy to convert carbon dioxide to fatty acid-derived chemicals that are widely used in the food and chemical industries. Herein, Synechococcus elongatus PCC 7942, a model cyanobacterium, was engineered for the first time to produce fatty acid ethyl esters (FAEEs) from CO₂. Due to the lack of an endogenous ethanol production pathway and wax ester synthase (AftA) activity in the wild-type cyanobacterium, we metabolically engineered S. elongatus PCC 7942 by expressing heterologous AftA and introducing the ethanol pathway, resulting in detectable peaks of FAEEs. To enhance FAEE production, a heterologous phosphoketolase pathway was introduced in the FAEE-producing strain to supply acetyl-CoA. Subsequent optimization of the cyanobacterial culture with a hexadecane overlay resulted in engineered S. elongatus PCC 7942 that produced photosynthetic FAEEs (10.0 ± 0.7 mg/L/OD₇₃₀) from CO₂. This paper is the first report of photosynthetic production of FAEEs from CO₂ in cyanobacteria.

Original language	English
Pages (from-to)	1087-1092
Number of pages	6
Journal	Journal of agricultural and food chemistry
Volume	65
Issue number	6
DOIs	https://doi.org/10.1021/acs.jafc.7b00002
Publication status	Published - 2017 Feb 15

Bibliographical note

Funding Information:
This work was financially supported by the Korea CCS R&D Center (KCRC) (Grant 2014M1A8A1049277) funded by the Korean government (Ministry of Science, Information and Communications Technology (ICT) & Future Planning). Also this work was partially supported by a Golden Seed Project (213008-05-1-WT911) grant funded by the Ministry of Agriculture, Ministry of Oceans and Fisheries.

Publisher Copyright:
© 2017 American Chemical Society.

Keywords

CO conversion
cyanobacteria
fatty acid ethyl ester
metabolic engineering

ASJC Scopus subject areas

Chemistry(all)
Agricultural and Biological Sciences(all)

Access to Document

10.1021/acs.jafc.7b00002

Cite this

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title = "Photosynthetic CO2 Conversion to Fatty Acid Ethyl Esters (FAEEs) Using Engineered Cyanobacteria",

abstract = "Metabolic engineering of cyanobacteria has received attention as a sustainable strategy to convert carbon dioxide to fatty acid-derived chemicals that are widely used in the food and chemical industries. Herein, Synechococcus elongatus PCC 7942, a model cyanobacterium, was engineered for the first time to produce fatty acid ethyl esters (FAEEs) from CO2. Due to the lack of an endogenous ethanol production pathway and wax ester synthase (AftA) activity in the wild-type cyanobacterium, we metabolically engineered S. elongatus PCC 7942 by expressing heterologous AftA and introducing the ethanol pathway, resulting in detectable peaks of FAEEs. To enhance FAEE production, a heterologous phosphoketolase pathway was introduced in the FAEE-producing strain to supply acetyl-CoA. Subsequent optimization of the cyanobacterial culture with a hexadecane overlay resulted in engineered S. elongatus PCC 7942 that produced photosynthetic FAEEs (10.0 ± 0.7 mg/L/OD730) from CO2. This paper is the first report of photosynthetic production of FAEEs from CO2 in cyanobacteria.",

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author = "Lee, {Hyun Jeong} and Jaeyeon Choi and Lee, {Sun Mi} and Youngsoon Um and Sim, {Sang Jun} and Yunje Kim and Woo, {Han Min}",

note = "Funding Information: This work was financially supported by the Korea CCS R&D Center (KCRC) (Grant 2014M1A8A1049277) funded by the Korean government (Ministry of Science, Information and Communications Technology (ICT) & Future Planning). Also this work was partially supported by a Golden Seed Project (213008-05-1-WT911) grant funded by the Ministry of Agriculture, Ministry of Oceans and Fisheries. Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

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AU - Sim, Sang Jun

AU - Kim, Yunje

AU - Woo, Han Min

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N2 - Metabolic engineering of cyanobacteria has received attention as a sustainable strategy to convert carbon dioxide to fatty acid-derived chemicals that are widely used in the food and chemical industries. Herein, Synechococcus elongatus PCC 7942, a model cyanobacterium, was engineered for the first time to produce fatty acid ethyl esters (FAEEs) from CO2. Due to the lack of an endogenous ethanol production pathway and wax ester synthase (AftA) activity in the wild-type cyanobacterium, we metabolically engineered S. elongatus PCC 7942 by expressing heterologous AftA and introducing the ethanol pathway, resulting in detectable peaks of FAEEs. To enhance FAEE production, a heterologous phosphoketolase pathway was introduced in the FAEE-producing strain to supply acetyl-CoA. Subsequent optimization of the cyanobacterial culture with a hexadecane overlay resulted in engineered S. elongatus PCC 7942 that produced photosynthetic FAEEs (10.0 ± 0.7 mg/L/OD730) from CO2. This paper is the first report of photosynthetic production of FAEEs from CO2 in cyanobacteria.

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