Phylogenetic analysis and in vitro bifunctional nuclease assay of arabidopsis BBD1 and BBD2

A. K.M. Mahmudul Huque, Won Mi So, Min Kyoung You, Jeong Sheop Shin

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Nucleases are a very diverse group of enzymes that play important roles in many crucial physiological processes in plants. We previously reported that the highly conserved region (HCR), domain of unknown function 151 (DUF151) and UV responsive (UVR) domain-containing OmBBD is a novel nuclease that does not share homology with other well-studied plant nucleases. Here, we report that DUF151 domain-containing proteins are present in bacteria, archaea and only Viridiplantae kingdom of eukarya, but not in any other eukaryotes. Two Arabidopsis homologs of OmBBD, AtBBD1 and AtBBD2, shared 43.69% and 44.38% sequence identity and contained all three distinct domains of OmBBD. We confirmed that the recombinant MBP-AtBBD1 and MBP-AtBBD2 exhibited non-substrate-specific DNase and RNase activity, like OmBBD. We also found that a metal cofactor is not necessarily required for DNase activity of AtBBD1 and AtBBD2, but their activities were much enhanced in the presence of Mg2+ or Mn2+. Using a yeast two-hybrid assay, we found that AtBBD1 and AtBBD2 each form a homodimer but not a heterodimer and that the HCR domain is possibly crucial for dimerization.

Original languageEnglish
Article number2169
Issue number9
Publication statusPublished - 2020 May

Bibliographical note

Funding Information:
Funding: This work was supported by a grant (2019R1F1A1060014) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and by a grant (PJ01367001) from the Next-Generation BioGreen 21 Program funded by the Rural Development Administration, Republic of Korea. This work was also partially supported by Korea University.

Publisher Copyright:
© 2020 by the authors.


  • Arabidopsis
  • AtBBD1
  • DUF151
  • Nuclease
  • OmBBD

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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