Phylogeographic diversity and hybrid zone of hantaan orthohantavirus collected in Gangwon Province, Republic of Korea

Geum Young Lee; Won Keun Kim; Kyungmin Park; Seung Ho Lee; Jusun Hwang; Jin Sun No; Seungchan Cho; Daesang Lee; Dong Hyun Song; Se Hun Gu; Man Seong Park; Seong Tae Jeong; Young Su Kim; Jin Won Song

doi:10.1371/journal.pntd.0008714

Phylogeographic diversity and hybrid zone of hantaan orthohantavirus collected in Gangwon Province, Republic of Korea

Geum Young Lee, Won Keun Kim, Kyungmin Park, Seung Ho Lee, Jusun Hwang, Jin Sun No, Seungchan Cho, Daesang Lee, Dong Hyun Song, Se Hun Gu, Man Seong Park, Seong Tae Jeong, Young Su Kim, Jin Won Song

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background Hantaan orthohantavirus (Hantaan virus, HTNV), harbored by Apodemus agrarius (the striped field mouse), causes hemorrhagic fever with renal syndrome (HFRS) in humans. Viral genome-based surveillance at new expansion sites to identify HFRS risks plays a criti-cal role in tracking the infection source of orthohantavirus outbreak. In the Republic of Korea (ROK), most studies demonstrated the serological prevalence and genetic diversity of ortho-hantaviruses collected from HFRS patients or rodents in Gyeonggi Province. Gangwon Province is a HFRS-endemic area with a high incidence of patients and prevalence of infected rodents, ROK. However, the continued epidemiology and surveillance of orthohan-tavirus remain to be investigated. Methodology/Principal findings Whole-genome sequencing of HTNV was accomplished in small mammals collected in Gangwon Province during 2015–2018 by multiplex PCR-based next-generation sequencing. To elucidate the geographic distribution and molecular diversity of viruses, we conducted phylogenetic analyses of HTNV tripartite genomes. We inferred the hybrid zone using cline analysis to estimate the geographic contact between two different HTNV lineages in the ROK. The graph incompatibility based reassortment finder performed reassortment analysis. A total of 12 HTNV genome sequences were completely obtained from A. agrarius newly collected in Gangwon Province. The phylogenetic and cline analyses demonstrated the genetic diversity and hybrid zone of HTNV in the ROK. Genetic exchange analysis suggested the possibility of reassortments in Cheorwon-gun, a highly HFRS-endemic area. Conclusions/Significance The prevalence and distribution of HTNV in HFRS-endemic areas of Gangwon Province enhanced the phylogeographic map for orthohantavirus outbreak monitoring in ROK. This study revealed the hybrid zone reflecting the genetic diversity and evolutionary dynamics of HTNV circulating in Gangwon Province. The results arise awareness of rodent-borne ortho-hantavirus diseases for physicians in the endemic area of ROK.

Original language	English
Article number	e0008714
Pages (from-to)	1-18
Number of pages	18
Journal	PLoS Neglected Tropical Diseases
Volume	14
Issue number	10
DOIs	https://doi.org/10.1371/journal.pntd.0008714
Publication status	Published - 2020 Oct

Bibliographical note

Funding Information:
This work was supported by the Research Program to Solve Social Issues of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Information and Communication Technology (ICT) (NRF-2017M3A9E4061992), the Agency for Defense Development (UE202026GD) and the Institute of Biomedical Science and Food Safety (IBF) (K2009831). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2020 Lee et al.

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1371/journal.pntd.0008714

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Lee, G. Y., Kim, W. K., Park, K., Lee, S. H., Hwang, J., No, J. S., Cho, S., Lee, D., Song, D. H., Gu, S. H., Park, M. S., Jeong, S. T., Kim, Y. S., & Song, J. W. (2020). Phylogeographic diversity and hybrid zone of hantaan orthohantavirus collected in Gangwon Province, Republic of Korea. PLoS Neglected Tropical Diseases, 14(10), 1-18. Article e0008714. https://doi.org/10.1371/journal.pntd.0008714

Lee, GY, Kim, WK, Park, K, Lee, SH, Hwang, J, No, JS, Cho, S, Lee, D, Song, DH, Gu, SH, Park, MS, Jeong, ST, Kim, YS & Song, JW 2020, 'Phylogeographic diversity and hybrid zone of hantaan orthohantavirus collected in Gangwon Province, Republic of Korea', PLoS Neglected Tropical Diseases, vol. 14, no. 10, e0008714, pp. 1-18. https://doi.org/10.1371/journal.pntd.0008714

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title = "Phylogeographic diversity and hybrid zone of hantaan orthohantavirus collected in Gangwon Province, Republic of Korea",

abstract = "Background Hantaan orthohantavirus (Hantaan virus, HTNV), harbored by Apodemus agrarius (the striped field mouse), causes hemorrhagic fever with renal syndrome (HFRS) in humans. Viral genome-based surveillance at new expansion sites to identify HFRS risks plays a criti-cal role in tracking the infection source of orthohantavirus outbreak. In the Republic of Korea (ROK), most studies demonstrated the serological prevalence and genetic diversity of ortho-hantaviruses collected from HFRS patients or rodents in Gyeonggi Province. Gangwon Province is a HFRS-endemic area with a high incidence of patients and prevalence of infected rodents, ROK. However, the continued epidemiology and surveillance of orthohan-tavirus remain to be investigated. Methodology/Principal findings Whole-genome sequencing of HTNV was accomplished in small mammals collected in Gangwon Province during 2015–2018 by multiplex PCR-based next-generation sequencing. To elucidate the geographic distribution and molecular diversity of viruses, we conducted phylogenetic analyses of HTNV tripartite genomes. We inferred the hybrid zone using cline analysis to estimate the geographic contact between two different HTNV lineages in the ROK. The graph incompatibility based reassortment finder performed reassortment analysis. A total of 12 HTNV genome sequences were completely obtained from A. agrarius newly collected in Gangwon Province. The phylogenetic and cline analyses demonstrated the genetic diversity and hybrid zone of HTNV in the ROK. Genetic exchange analysis suggested the possibility of reassortments in Cheorwon-gun, a highly HFRS-endemic area. Conclusions/Significance The prevalence and distribution of HTNV in HFRS-endemic areas of Gangwon Province enhanced the phylogeographic map for orthohantavirus outbreak monitoring in ROK. This study revealed the hybrid zone reflecting the genetic diversity and evolutionary dynamics of HTNV circulating in Gangwon Province. The results arise awareness of rodent-borne ortho-hantavirus diseases for physicians in the endemic area of ROK.",

author = "Lee, {Geum Young} and Kim, {Won Keun} and Kyungmin Park and Lee, {Seung Ho} and Jusun Hwang and No, {Jin Sun} and Seungchan Cho and Daesang Lee and Song, {Dong Hyun} and Gu, {Se Hun} and Park, {Man Seong} and Jeong, {Seong Tae} and Kim, {Young Su} and Song, {Jin Won}",

note = "Funding Information: This work was supported by the Research Program to Solve Social Issues of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Information and Communication Technology (ICT) (NRF-2017M3A9E4061992), the Agency for Defense Development (UE202026GD) and the Institute of Biomedical Science and Food Safety (IBF) (K2009831). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2020 Lee et al.",

year = "2020",

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T1 - Phylogeographic diversity and hybrid zone of hantaan orthohantavirus collected in Gangwon Province, Republic of Korea

AU - Lee, Geum Young

AU - Kim, Won Keun

AU - Park, Kyungmin

AU - Lee, Seung Ho

AU - Hwang, Jusun

AU - No, Jin Sun

AU - Cho, Seungchan

AU - Lee, Daesang

AU - Song, Dong Hyun

AU - Gu, Se Hun

AU - Park, Man Seong

AU - Jeong, Seong Tae

AU - Kim, Young Su

AU - Song, Jin Won

N1 - Funding Information: This work was supported by the Research Program to Solve Social Issues of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Information and Communication Technology (ICT) (NRF-2017M3A9E4061992), the Agency for Defense Development (UE202026GD) and the Institute of Biomedical Science and Food Safety (IBF) (K2009831). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2020 Lee et al.

PY - 2020/10

Y1 - 2020/10

N2 - Background Hantaan orthohantavirus (Hantaan virus, HTNV), harbored by Apodemus agrarius (the striped field mouse), causes hemorrhagic fever with renal syndrome (HFRS) in humans. Viral genome-based surveillance at new expansion sites to identify HFRS risks plays a criti-cal role in tracking the infection source of orthohantavirus outbreak. In the Republic of Korea (ROK), most studies demonstrated the serological prevalence and genetic diversity of ortho-hantaviruses collected from HFRS patients or rodents in Gyeonggi Province. Gangwon Province is a HFRS-endemic area with a high incidence of patients and prevalence of infected rodents, ROK. However, the continued epidemiology and surveillance of orthohan-tavirus remain to be investigated. Methodology/Principal findings Whole-genome sequencing of HTNV was accomplished in small mammals collected in Gangwon Province during 2015–2018 by multiplex PCR-based next-generation sequencing. To elucidate the geographic distribution and molecular diversity of viruses, we conducted phylogenetic analyses of HTNV tripartite genomes. We inferred the hybrid zone using cline analysis to estimate the geographic contact between two different HTNV lineages in the ROK. The graph incompatibility based reassortment finder performed reassortment analysis. A total of 12 HTNV genome sequences were completely obtained from A. agrarius newly collected in Gangwon Province. The phylogenetic and cline analyses demonstrated the genetic diversity and hybrid zone of HTNV in the ROK. Genetic exchange analysis suggested the possibility of reassortments in Cheorwon-gun, a highly HFRS-endemic area. Conclusions/Significance The prevalence and distribution of HTNV in HFRS-endemic areas of Gangwon Province enhanced the phylogeographic map for orthohantavirus outbreak monitoring in ROK. This study revealed the hybrid zone reflecting the genetic diversity and evolutionary dynamics of HTNV circulating in Gangwon Province. The results arise awareness of rodent-borne ortho-hantavirus diseases for physicians in the endemic area of ROK.

AB - Background Hantaan orthohantavirus (Hantaan virus, HTNV), harbored by Apodemus agrarius (the striped field mouse), causes hemorrhagic fever with renal syndrome (HFRS) in humans. Viral genome-based surveillance at new expansion sites to identify HFRS risks plays a criti-cal role in tracking the infection source of orthohantavirus outbreak. In the Republic of Korea (ROK), most studies demonstrated the serological prevalence and genetic diversity of ortho-hantaviruses collected from HFRS patients or rodents in Gyeonggi Province. Gangwon Province is a HFRS-endemic area with a high incidence of patients and prevalence of infected rodents, ROK. However, the continued epidemiology and surveillance of orthohan-tavirus remain to be investigated. Methodology/Principal findings Whole-genome sequencing of HTNV was accomplished in small mammals collected in Gangwon Province during 2015–2018 by multiplex PCR-based next-generation sequencing. To elucidate the geographic distribution and molecular diversity of viruses, we conducted phylogenetic analyses of HTNV tripartite genomes. We inferred the hybrid zone using cline analysis to estimate the geographic contact between two different HTNV lineages in the ROK. The graph incompatibility based reassortment finder performed reassortment analysis. A total of 12 HTNV genome sequences were completely obtained from A. agrarius newly collected in Gangwon Province. The phylogenetic and cline analyses demonstrated the genetic diversity and hybrid zone of HTNV in the ROK. Genetic exchange analysis suggested the possibility of reassortments in Cheorwon-gun, a highly HFRS-endemic area. Conclusions/Significance The prevalence and distribution of HTNV in HFRS-endemic areas of Gangwon Province enhanced the phylogeographic map for orthohantavirus outbreak monitoring in ROK. This study revealed the hybrid zone reflecting the genetic diversity and evolutionary dynamics of HTNV circulating in Gangwon Province. The results arise awareness of rodent-borne ortho-hantavirus diseases for physicians in the endemic area of ROK.

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Phylogeographic diversity and hybrid zone of hantaan orthohantavirus collected in Gangwon Province, Republic of Korea

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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