Physalis alkekengi L. var. francheti alleviates neuronal cell death caused by activated microglia in vitro

Byoung Hee Park; Oh Wook Kwon; In Sung Kim; Hae Min Lee; Yeon Jin Roh; Minseo Kim; Youngho Jo; Hwayeon Cho; Jung Kuk Park; Zheng Zhi; Byung Cheon Lee

doi:10.1186/s13765-021-00594-6

Physalis alkekengi L. var. francheti alleviates neuronal cell death caused by activated microglia in vitro

Byoung Hee Park, Oh Wook Kwon, In Sung Kim, Hae Min Lee, Yeon Jin Roh, Minseo Kim, Youngho Jo, Hwayeon Cho, Jung Kuk Park, Zheng Zhi, Byung Cheon Lee

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Microglia are the macrophages that reside in the brain. Activated microglia induces further activation of astrocytes and neuronal cells for mounting an immune response. However, activated microglia release neurotoxic mediators causing neuroinflammation, which is associated with chronic etiology of neurodegenerative diseases. We investigated the effect of ethanol extract of Physalis alkekengi L. var. francheti fruit (PAFE) on neuronal cell death mediated by activated microglia. PAFE decreased NO production and IL-6 secretion in LPS-stimulated BV-2 and primary microglial cells without reducing cell viability. Consistently, treatment with PAFE decreased iNOS and COX-2 expression and ERK phosphorylation in LPS-stimulated BV-2 cells. Finally, apoptosis of N2a cells grown in conditioned media prepared from LPS-stimulated BV-2 cells containing PAFE was inhibited via downregulation of the Bax/Bcl-2 ratio. Taken together, PAFE alleviates neuronal cell death by reducing neurotoxic mediators such as NO and IL-6 from activated microglia. Therefore, it could be a potential candidate to treat neurodegenerative diseases caused by chronic neuroinflammation.

Original language	English
Article number	23
Journal	Applied Biological Chemistry
Volume	64
Issue number	1
DOIs	https://doi.org/10.1186/s13765-021-00594-6
Publication status	Published - 2021 Dec

Bibliographical note

Publisher Copyright:
© 2021, The Author(s).

Keywords

BV-2
Microglia
N2a
Neuroinflammation
Neuroprotection
Physalis alkekengi

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
Organic Chemistry

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title = "Physalis alkekengi L. var. francheti alleviates neuronal cell death caused by activated microglia in vitro",

abstract = "Microglia are the macrophages that reside in the brain. Activated microglia induces further activation of astrocytes and neuronal cells for mounting an immune response. However, activated microglia release neurotoxic mediators causing neuroinflammation, which is associated with chronic etiology of neurodegenerative diseases. We investigated the effect of ethanol extract of Physalis alkekengi L. var. francheti fruit (PAFE) on neuronal cell death mediated by activated microglia. PAFE decreased NO production and IL-6 secretion in LPS-stimulated BV-2 and primary microglial cells without reducing cell viability. Consistently, treatment with PAFE decreased iNOS and COX-2 expression and ERK phosphorylation in LPS-stimulated BV-2 cells. Finally, apoptosis of N2a cells grown in conditioned media prepared from LPS-stimulated BV-2 cells containing PAFE was inhibited via downregulation of the Bax/Bcl-2 ratio. Taken together, PAFE alleviates neuronal cell death by reducing neurotoxic mediators such as NO and IL-6 from activated microglia. Therefore, it could be a potential candidate to treat neurodegenerative diseases caused by chronic neuroinflammation.",

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author = "Park, {Byoung Hee} and Kwon, {Oh Wook} and Kim, {In Sung} and Lee, {Hae Min} and Roh, {Yeon Jin} and Minseo Kim and Youngho Jo and Hwayeon Cho and Park, {Jung Kuk} and Zheng Zhi and Lee, {Byung Cheon}",

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year = "2021",

month = dec,

doi = "10.1186/s13765-021-00594-6",

language = "English",

volume = "64",

journal = "Applied Biological Chemistry",

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T1 - Physalis alkekengi L. var. francheti alleviates neuronal cell death caused by activated microglia in vitro

AU - Park, Byoung Hee

AU - Kwon, Oh Wook

AU - Kim, In Sung

AU - Lee, Hae Min

AU - Roh, Yeon Jin

AU - Kim, Minseo

AU - Jo, Youngho

AU - Cho, Hwayeon

AU - Park, Jung Kuk

AU - Zhi, Zheng

AU - Lee, Byung Cheon

PY - 2021/12

Y1 - 2021/12

N2 - Microglia are the macrophages that reside in the brain. Activated microglia induces further activation of astrocytes and neuronal cells for mounting an immune response. However, activated microglia release neurotoxic mediators causing neuroinflammation, which is associated with chronic etiology of neurodegenerative diseases. We investigated the effect of ethanol extract of Physalis alkekengi L. var. francheti fruit (PAFE) on neuronal cell death mediated by activated microglia. PAFE decreased NO production and IL-6 secretion in LPS-stimulated BV-2 and primary microglial cells without reducing cell viability. Consistently, treatment with PAFE decreased iNOS and COX-2 expression and ERK phosphorylation in LPS-stimulated BV-2 cells. Finally, apoptosis of N2a cells grown in conditioned media prepared from LPS-stimulated BV-2 cells containing PAFE was inhibited via downregulation of the Bax/Bcl-2 ratio. Taken together, PAFE alleviates neuronal cell death by reducing neurotoxic mediators such as NO and IL-6 from activated microglia. Therefore, it could be a potential candidate to treat neurodegenerative diseases caused by chronic neuroinflammation.

AB - Microglia are the macrophages that reside in the brain. Activated microglia induces further activation of astrocytes and neuronal cells for mounting an immune response. However, activated microglia release neurotoxic mediators causing neuroinflammation, which is associated with chronic etiology of neurodegenerative diseases. We investigated the effect of ethanol extract of Physalis alkekengi L. var. francheti fruit (PAFE) on neuronal cell death mediated by activated microglia. PAFE decreased NO production and IL-6 secretion in LPS-stimulated BV-2 and primary microglial cells without reducing cell viability. Consistently, treatment with PAFE decreased iNOS and COX-2 expression and ERK phosphorylation in LPS-stimulated BV-2 cells. Finally, apoptosis of N2a cells grown in conditioned media prepared from LPS-stimulated BV-2 cells containing PAFE was inhibited via downregulation of the Bax/Bcl-2 ratio. Taken together, PAFE alleviates neuronal cell death by reducing neurotoxic mediators such as NO and IL-6 from activated microglia. Therefore, it could be a potential candidate to treat neurodegenerative diseases caused by chronic neuroinflammation.

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KW - Neuroprotection

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ER -

Physalis alkekengi L. var. francheti alleviates neuronal cell death caused by activated microglia in vitro

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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