Pioneer round of translation mediated by nuclear cap-binding proteins CBP80/20 occurs during prolonged hypoxia

Nara Oh; Kyoung Mi Kim; Junho Choe; Yoon Ki Kim

doi:10.1016/j.febslet.2007.10.002

Pioneer round of translation mediated by nuclear cap-binding proteins CBP80/20 occurs during prolonged hypoxia

Nara Oh, Kyoung Mi Kim, Junho Choe, Yoon Ki Kim

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Nonsense-mediated mRNA decay (NMD) is one of the mRNA surveillance mechanisms, which eliminates aberrant mRNAs harboring premature termination codons. NMD targets only mRNAs bound by the nuclear cap-binding protein complex CBP80/20 which directs the pioneer round of translation. Here we demonstrate that NMD occurs efficiently during prolonged hypoxia in which steady-state translation is drastically inhibited. Accordingly, CBP80 remains in the nucleus, and processing bodies are unaffected with regard to their abundance and number under prolonged hypoxic conditions. These results indicate that mRNAs enter the pioneer round of translation during prolonged hypoxia.

Original language	English
Pages (from-to)	5158-5164
Number of pages	7
Journal	FEBS Letters
Volume	581
Issue number	26
DOIs	https://doi.org/10.1016/j.febslet.2007.10.002
Publication status	Published - 2007 Oct 30

Bibliographical note

Funding Information:
We thank Lynne E. Maquat for providing the NMD reporter constructs and α-Upfs antibodies, Robin Reed for α-CBP80 antibody and α-eIF4AIII antibody, John W. Hershey for α-eIF3 antibody, Sung-Gil Chi for α-HIF-1α antibody, Sung Key Jang for α-eIF4GI antibody, and Jens Lykke-Andersen for pCDNA3-FLAG-Dcp1a. This work was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A062356), a Grant (20070301034003) from BioGreen 21 Program, Rural Development Administration, Republic of Korea, and the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (R01-2006-000-10194-0).

Keywords

Eukaryotic translation initiation factor
Hypoxia
Nonsense-mediated mRNA decay
Nuclear cap-binding protein CBP80/20
Pioneer round of translation

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.10.002

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title = "Pioneer round of translation mediated by nuclear cap-binding proteins CBP80/20 occurs during prolonged hypoxia",

abstract = "Nonsense-mediated mRNA decay (NMD) is one of the mRNA surveillance mechanisms, which eliminates aberrant mRNAs harboring premature termination codons. NMD targets only mRNAs bound by the nuclear cap-binding protein complex CBP80/20 which directs the pioneer round of translation. Here we demonstrate that NMD occurs efficiently during prolonged hypoxia in which steady-state translation is drastically inhibited. Accordingly, CBP80 remains in the nucleus, and processing bodies are unaffected with regard to their abundance and number under prolonged hypoxic conditions. These results indicate that mRNAs enter the pioneer round of translation during prolonged hypoxia.",

keywords = "Eukaryotic translation initiation factor, Hypoxia, Nonsense-mediated mRNA decay, Nuclear cap-binding protein CBP80/20, Pioneer round of translation",

author = "Nara Oh and Kim, {Kyoung Mi} and Junho Choe and Kim, {Yoon Ki}",

note = "Funding Information: We thank Lynne E. Maquat for providing the NMD reporter constructs and α-Upfs antibodies, Robin Reed for α-CBP80 antibody and α-eIF4AIII antibody, John W. Hershey for α-eIF3 antibody, Sung-Gil Chi for α-HIF-1α antibody, Sung Key Jang for α-eIF4GI antibody, and Jens Lykke-Andersen for pCDNA3-FLAG-Dcp1a. This work was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A062356), a Grant (20070301034003) from BioGreen 21 Program, Rural Development Administration, Republic of Korea, and the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (R01-2006-000-10194-0).",

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AU - Oh, Nara

AU - Kim, Kyoung Mi

AU - Choe, Junho

AU - Kim, Yoon Ki

N1 - Funding Information: We thank Lynne E. Maquat for providing the NMD reporter constructs and α-Upfs antibodies, Robin Reed for α-CBP80 antibody and α-eIF4AIII antibody, John W. Hershey for α-eIF3 antibody, Sung-Gil Chi for α-HIF-1α antibody, Sung Key Jang for α-eIF4GI antibody, and Jens Lykke-Andersen for pCDNA3-FLAG-Dcp1a. This work was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A062356), a Grant (20070301034003) from BioGreen 21 Program, Rural Development Administration, Republic of Korea, and the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (R01-2006-000-10194-0).

PY - 2007/10/30

Y1 - 2007/10/30

N2 - Nonsense-mediated mRNA decay (NMD) is one of the mRNA surveillance mechanisms, which eliminates aberrant mRNAs harboring premature termination codons. NMD targets only mRNAs bound by the nuclear cap-binding protein complex CBP80/20 which directs the pioneer round of translation. Here we demonstrate that NMD occurs efficiently during prolonged hypoxia in which steady-state translation is drastically inhibited. Accordingly, CBP80 remains in the nucleus, and processing bodies are unaffected with regard to their abundance and number under prolonged hypoxic conditions. These results indicate that mRNAs enter the pioneer round of translation during prolonged hypoxia.

AB - Nonsense-mediated mRNA decay (NMD) is one of the mRNA surveillance mechanisms, which eliminates aberrant mRNAs harboring premature termination codons. NMD targets only mRNAs bound by the nuclear cap-binding protein complex CBP80/20 which directs the pioneer round of translation. Here we demonstrate that NMD occurs efficiently during prolonged hypoxia in which steady-state translation is drastically inhibited. Accordingly, CBP80 remains in the nucleus, and processing bodies are unaffected with regard to their abundance and number under prolonged hypoxic conditions. These results indicate that mRNAs enter the pioneer round of translation during prolonged hypoxia.

KW - Eukaryotic translation initiation factor

KW - Hypoxia

KW - Nonsense-mediated mRNA decay

KW - Nuclear cap-binding protein CBP80/20

KW - Pioneer round of translation

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AN - SCOPUS:35348909673

SN - 0014-5793

VL - 581

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JO - FEBS Letters

JF - FEBS Letters

IS - 26

ER -

Pioneer round of translation mediated by nuclear cap-binding proteins CBP80/20 occurs during prolonged hypoxia

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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