Pioneer round of translation occurs during serum starvation

Nara Oh; Kyoung Mi Kim; Hana Cho; Junho Choe; Yoon Ki Kim

doi:10.1016/j.bbrc.2007.07.169

Pioneer round of translation occurs during serum starvation

Nara Oh, Kyoung Mi Kim, Hana Cho, Junho Choe, Yoon Ki Kim

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The pioneer round of translation plays a role in translation initiation of newly spliced and exon junction complex (EJC)-bound mRNAs. Nuclear cap-binding protein complex CBP80/20 binds to those mRNAs at the 5′-end, recruiting translation initiation complex. As a consequence of the pioneer round of translation, the bound EJCs are dissociated from mRNAs and CBP80/20 is replaced by the cytoplasmic cap-binding protein eIF4E. Steady-state translation directed by eIF4E allows for an immediate and rapid response to changes in physiological conditions. Here, we show that nonsense-mediated mRNA decay (NMD), which restricts only to the pioneer round of translation but not to steady-state translation, efficiently occurs even during serum starvation, in which steady-state translation is drastically abolished. Accordingly, CBP80 remains in the nucleus and processing bodies are unaffected in their abundance and number in serum-starved conditions. These results suggest that mRNAs enter the pioneer round of translation during serum starvation and are targeted for NMD if they contain premature termination codons.

Original language	English
Pages (from-to)	145-151
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	362
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2007.07.169
Publication status	Published - 2007 Oct 12

Bibliographical note

Funding Information:
We thank Lynne E. Maquat for providing NMD reporter constructs and α-Upf antibodies, Robin Reed for α-CBP80 antibody and α-eIF4AIII antibody, John W. Hershey for α-eIF3 antibody, Sung Key Jang for α-eIF4GI antibody, and Jens Lykke-Andersen for pCDNA3-FLAG-Dcp1a. We also thank Incheol Ryu for helpful comments. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD)” (KRF-2006-311-E00204), a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A062356), and a grant (20070301034003) from BioGreen 21 Program, Rural Development Administration, Republic of Korea.

Keywords

CBP80
Nonsense-mediated mRNA decay
Pioneer round of translation
Processing body
Serum starvation
Steady-state translation
Stress granule
eIF4E

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2007.07.169

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@article{c48cadd06c9a4b56b85416073159166d,

title = "Pioneer round of translation occurs during serum starvation",

abstract = "The pioneer round of translation plays a role in translation initiation of newly spliced and exon junction complex (EJC)-bound mRNAs. Nuclear cap-binding protein complex CBP80/20 binds to those mRNAs at the 5′-end, recruiting translation initiation complex. As a consequence of the pioneer round of translation, the bound EJCs are dissociated from mRNAs and CBP80/20 is replaced by the cytoplasmic cap-binding protein eIF4E. Steady-state translation directed by eIF4E allows for an immediate and rapid response to changes in physiological conditions. Here, we show that nonsense-mediated mRNA decay (NMD), which restricts only to the pioneer round of translation but not to steady-state translation, efficiently occurs even during serum starvation, in which steady-state translation is drastically abolished. Accordingly, CBP80 remains in the nucleus and processing bodies are unaffected in their abundance and number in serum-starved conditions. These results suggest that mRNAs enter the pioneer round of translation during serum starvation and are targeted for NMD if they contain premature termination codons.",

keywords = "CBP80, Nonsense-mediated mRNA decay, Pioneer round of translation, Processing body, Serum starvation, Steady-state translation, Stress granule, eIF4E",

author = "Nara Oh and Kim, {Kyoung Mi} and Hana Cho and Junho Choe and Kim, {Yoon Ki}",

note = "Funding Information: We thank Lynne E. Maquat for providing NMD reporter constructs and α-Upf antibodies, Robin Reed for α-CBP80 antibody and α-eIF4AIII antibody, John W. Hershey for α-eIF3 antibody, Sung Key Jang for α-eIF4GI antibody, and Jens Lykke-Andersen for pCDNA3-FLAG-Dcp1a. We also thank Incheol Ryu for helpful comments. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD)” (KRF-2006-311-E00204), a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A062356), and a grant (20070301034003) from BioGreen 21 Program, Rural Development Administration, Republic of Korea.",

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AU - Oh, Nara

AU - Kim, Kyoung Mi

AU - Cho, Hana

AU - Choe, Junho

AU - Kim, Yoon Ki

N1 - Funding Information: We thank Lynne E. Maquat for providing NMD reporter constructs and α-Upf antibodies, Robin Reed for α-CBP80 antibody and α-eIF4AIII antibody, John W. Hershey for α-eIF3 antibody, Sung Key Jang for α-eIF4GI antibody, and Jens Lykke-Andersen for pCDNA3-FLAG-Dcp1a. We also thank Incheol Ryu for helpful comments. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD)” (KRF-2006-311-E00204), a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A062356), and a grant (20070301034003) from BioGreen 21 Program, Rural Development Administration, Republic of Korea.

PY - 2007/10/12

Y1 - 2007/10/12

N2 - The pioneer round of translation plays a role in translation initiation of newly spliced and exon junction complex (EJC)-bound mRNAs. Nuclear cap-binding protein complex CBP80/20 binds to those mRNAs at the 5′-end, recruiting translation initiation complex. As a consequence of the pioneer round of translation, the bound EJCs are dissociated from mRNAs and CBP80/20 is replaced by the cytoplasmic cap-binding protein eIF4E. Steady-state translation directed by eIF4E allows for an immediate and rapid response to changes in physiological conditions. Here, we show that nonsense-mediated mRNA decay (NMD), which restricts only to the pioneer round of translation but not to steady-state translation, efficiently occurs even during serum starvation, in which steady-state translation is drastically abolished. Accordingly, CBP80 remains in the nucleus and processing bodies are unaffected in their abundance and number in serum-starved conditions. These results suggest that mRNAs enter the pioneer round of translation during serum starvation and are targeted for NMD if they contain premature termination codons.

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ER -

Pioneer round of translation occurs during serum starvation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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