Plasma proteomic analysis of patients infected with H1N1 influenza virus

Seonyoung Choi, Ji youn Lim, Yoonjin Kim, Moon Jung Song, Woon Won Jung, Jung Bok Seo, Jacob Lee, Donggeun Sul

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)

    Abstract

    This study profiled the plasma proteins of patients infected by the 2011 H1N1 influenza virus. Differential protein expression was identified in plasma obtained from noninfected control subjects (n = 15) and H1N1-infected subjects (n = 15). Plasma proteins were separated by a 2DE large gel system and identified by nano-ultra performance LC-MS. Western blot assays were performed to validate proteins. Eight plasma proteins were upregulated and six proteins were downregulated among 3316 plasma proteins in the H1N1-infected group as compared with the control group. Of 14 up- and downregulated proteins, nine plasma proteins were validated by Western blot analysis. Putative protein FAM 157A, leucine-rich alpha 2 glycoprotein, serum amyloid A protein, and dual oxidase 1 showed significant differential expression. The identified plasma proteins could be potential candidates for biomarkers of H1N1 influenza viral infection. Further studies are needed to develop these proteins as diagnostic biomarkers.

    Original languageEnglish
    Pages (from-to)1933-1942
    Number of pages10
    JournalProteomics
    Volume14
    Issue number16
    DOIs
    Publication statusPublished - 2014 Aug

    Keywords

    • Biomarkers
    • Biomedicine
    • H1N1
    • Influenza virus
    • Plasma proteins

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

    Fingerprint

    Dive into the research topics of 'Plasma proteomic analysis of patients infected with H1N1 influenza virus'. Together they form a unique fingerprint.

    Cite this