Plasmon resonance energy transfer sp\ectroscopy (PRET)

Yeonho Choi; Taewook Kang; Gang L. Liu; Yitao Long; Luke P. Lee

Plasmon resonance energy transfer sp\ectroscopy (PRET)

Yeonho Choi, Taewook Kang, Gang L. Liu, Yitao Long, Luke P. Lee

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We demonstrate that gold nanoparticles (GNPs) with self-assembled monolayer (SAM) can function as all-optical nanoscale biomolecular detectors that monitor in situ resonant biomolecules, such as Cytochrome c. By tethering resonant biomolecules to Au nanoprobes, the binding events of Cytochrome c onto 50-nm Au nanoprobes yield characteristic spectral dips in scattering spectra of Au nanoprobes due to plasmon resonance energy transfer (PRET). The quenching dip depth of the single nanoprobe spectrum is found to be a function of incubation time (t) and concentration of Cytochrome c, which provides a real-time and nanoscale measurement and quantification of Cytochrome c.

Original language	English
Title of host publication	Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Editors	Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
Publisher	Chemical and Biological Microsystems Society
Pages	1610-1612
Number of pages	3
ISBN (Electronic)	9780979806407
Publication status	Published - 2007
Externally published	Yes
Event	11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France Duration: 2007 Oct 7 → 2007 Oct 11

Publication series

Name	Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference	11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Country/Territory	France
City	Paris
Period	07/10/7 → 07/10/11

Bibliographical note

Funding Information:
This work was supported by a grant (code #: 05K1501-02810) from ‘Center for Nanostructured Materials Technology’ under ‘21st Century Frontier R&D Programs’ of the Ministry of Science and Technology, Korea.

Publisher Copyright:
© 2007 CBMS.

Keywords

Cytochrome C
Gold Nanoparticle
Plasmonic Resonance Energy Transfer
Rayleigh Scattering

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Choi, Y., Kang, T., Liu, G. L., Long, Y., & Lee, L. P. (2007). Plasmon resonance energy transfer sp\ectroscopy (PRET). In J.-L. Viovy, P. Tabeling, S. Descroix, & L. Malaquin (Eds.), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 (pp. 1610-1612). (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007). Chemical and Biological Microsystems Society.

Plasmon resonance energy transfer sp\ectroscopy (PRET). / Choi, Yeonho; Kang, Taewook; Liu, Gang L. et al.
Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. ed. / Jean-Louis Viovy; Patrick Tabeling; Stephanie Descroix; Laurent Malaquin. Chemical and Biological Microsystems Society, 2007. p. 1610-1612 (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Choi, Y, Kang, T, Liu, GL, Long, Y & Lee, LP 2007, Plasmon resonance energy transfer sp\ectroscopy (PRET). in J-L Viovy, P Tabeling, S Descroix & L Malaquin (eds), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007, Chemical and Biological Microsystems Society, pp. 1610-1612, 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007, Paris, France, 07/10/7.

Choi Y, Kang T, Liu GL, Long Y, Lee LP. Plasmon resonance energy transfer sp\ectroscopy (PRET). In Viovy JL, Tabeling P, Descroix S, Malaquin L, editors, Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. Chemical and Biological Microsystems Society. 2007. p. 1610-1612. (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007).

Choi, Yeonho ; Kang, Taewook ; Liu, Gang L. et al. / Plasmon resonance energy transfer sp\ectroscopy (PRET). Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. editor / Jean-Louis Viovy ; Patrick Tabeling ; Stephanie Descroix ; Laurent Malaquin. Chemical and Biological Microsystems Society, 2007. pp. 1610-1612 (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007).

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abstract = "We demonstrate that gold nanoparticles (GNPs) with self-assembled monolayer (SAM) can function as all-optical nanoscale biomolecular detectors that monitor in situ resonant biomolecules, such as Cytochrome c. By tethering resonant biomolecules to Au nanoprobes, the binding events of Cytochrome c onto 50-nm Au nanoprobes yield characteristic spectral dips in scattering spectra of Au nanoprobes due to plasmon resonance energy transfer (PRET). The quenching dip depth of the single nanoprobe spectrum is found to be a function of incubation time (t) and concentration of Cytochrome c, which provides a real-time and nanoscale measurement and quantification of Cytochrome c.",

keywords = "Cytochrome C, Gold Nanoparticle, Plasmonic Resonance Energy Transfer, Rayleigh Scattering",

author = "Yeonho Choi and Taewook Kang and Liu, {Gang L.} and Yitao Long and Lee, {Luke P.}",

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AU - Kang, Taewook

AU - Liu, Gang L.

AU - Long, Yitao

AU - Lee, Luke P.

N1 - Funding Information: This work was supported by a grant (code #: 05K1501-02810) from ‘Center for Nanostructured Materials Technology’ under ‘21st Century Frontier R&D Programs’ of the Ministry of Science and Technology, Korea. Publisher Copyright: © 2007 CBMS.

PY - 2007

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N2 - We demonstrate that gold nanoparticles (GNPs) with self-assembled monolayer (SAM) can function as all-optical nanoscale biomolecular detectors that monitor in situ resonant biomolecules, such as Cytochrome c. By tethering resonant biomolecules to Au nanoprobes, the binding events of Cytochrome c onto 50-nm Au nanoprobes yield characteristic spectral dips in scattering spectra of Au nanoprobes due to plasmon resonance energy transfer (PRET). The quenching dip depth of the single nanoprobe spectrum is found to be a function of incubation time (t) and concentration of Cytochrome c, which provides a real-time and nanoscale measurement and quantification of Cytochrome c.

AB - We demonstrate that gold nanoparticles (GNPs) with self-assembled monolayer (SAM) can function as all-optical nanoscale biomolecular detectors that monitor in situ resonant biomolecules, such as Cytochrome c. By tethering resonant biomolecules to Au nanoprobes, the binding events of Cytochrome c onto 50-nm Au nanoprobes yield characteristic spectral dips in scattering spectra of Au nanoprobes due to plasmon resonance energy transfer (PRET). The quenching dip depth of the single nanoprobe spectrum is found to be a function of incubation time (t) and concentration of Cytochrome c, which provides a real-time and nanoscale measurement and quantification of Cytochrome c.

KW - Cytochrome C

KW - Gold Nanoparticle

KW - Plasmonic Resonance Energy Transfer

KW - Rayleigh Scattering

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T3 - Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

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BT - Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

A2 - Viovy, Jean-Louis

A2 - Tabeling, Patrick

A2 - Descroix, Stephanie

A2 - Malaquin, Laurent

PB - Chemical and Biological Microsystems Society

T2 - 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

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ER -

Plasmon resonance energy transfer sp\ectroscopy (PRET)

Abstract

Publication series

Conference

Bibliographical note

Keywords

ASJC Scopus subject areas

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Cite this