Poly-γ-glutamic acid enhances the growth and viability of Chinese hamster ovary cells in serum-free medium.

Bok Hwan Chun; Yong Kwon Lee; Namhyun Chung

doi:10.1007/s10529-012-0982-8

Poly-γ-glutamic acid enhances the growth and viability of Chinese hamster ovary cells in serum-free medium.

Bok Hwan Chun, Yong Kwon Lee, Namhyun Chung

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection was dependent upon the molecular weight of γPGA and its concentration. When 0.05 or 0.075 % of 500 kDa γPGA was added, the cell growth and viability were almost equal to those of Pluronic F68 supplementation and were much higher than those of the control without additives. For the first time, we show that γPGA is another environmentally-friendly medium additive that can be used in place of Pluronic F68.

Original language	English
Pages (from-to)	1807-1810
Number of pages	4
Journal	Biotechnology letters
Volume	34
Issue number	10
DOIs	https://doi.org/10.1007/s10529-012-0982-8
Publication status	Published - 2012 Oct

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-012-0982-8

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title = "Poly-γ-glutamic acid enhances the growth and viability of Chinese hamster ovary cells in serum-free medium.",

abstract = "The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection was dependent upon the molecular weight of γPGA and its concentration. When 0.05 or 0.075 % of 500 kDa γPGA was added, the cell growth and viability were almost equal to those of Pluronic F68 supplementation and were much higher than those of the control without additives. For the first time, we show that γPGA is another environmentally-friendly medium additive that can be used in place of Pluronic F68.",

author = "Chun, {Bok Hwan} and Lee, {Yong Kwon} and Namhyun Chung",

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T1 - Poly-γ-glutamic acid enhances the growth and viability of Chinese hamster ovary cells in serum-free medium.

AU - Chun, Bok Hwan

AU - Lee, Yong Kwon

AU - Chung, Namhyun

N1 - Funding Information: This study was supported by Korea Sanhak Foundation, Republic of Korea.

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Y1 - 2012/10

N2 - The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection was dependent upon the molecular weight of γPGA and its concentration. When 0.05 or 0.075 % of 500 kDa γPGA was added, the cell growth and viability were almost equal to those of Pluronic F68 supplementation and were much higher than those of the control without additives. For the first time, we show that γPGA is another environmentally-friendly medium additive that can be used in place of Pluronic F68.

AB - The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection was dependent upon the molecular weight of γPGA and its concentration. When 0.05 or 0.075 % of 500 kDa γPGA was added, the cell growth and viability were almost equal to those of Pluronic F68 supplementation and were much higher than those of the control without additives. For the first time, we show that γPGA is another environmentally-friendly medium additive that can be used in place of Pluronic F68.

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Poly-γ-glutamic acid enhances the growth and viability of Chinese hamster ovary cells in serum-free medium.

Abstract

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