Poly-γ-glutamic acid enhances the quality of recombant erythropoietin produced by CHO cells

Tae Gon Kim; Young Chol Cho; Bok Hwan Chun; Sung Hyo Park; Hoi Seon Lee; Namhyun Chung

doi:10.1080/13102818.2014.901675

Poly-γ-glutamic acid enhances the quality of recombant erythropoietin produced by CHO cells

Tae Gon Kim, Young Chol Cho, Bok Hwan Chun, Sung Hyo Park, Hoi Seon Lee, Namhyun Chung

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of poly-γ-glutamic acid (γPGA), which is produced by Bacillus sp., on the production of recombinant erythropoietin (rEPO) by Chinese hamster ovary (CHO) cells in suspension culture was evaluated. The growth, viability, and productivity of recombinant CHO cells were investigated in a chemically defined medium with 50 and 500 kD γPGAs at 0.075% or with Pluronic F68. Cell growth with the two γPGAs was lower than that with Pluronic F68 but significantly higher than that without any additive (control). The effect of additives on rEPO productivity was 50 kDa γPGA > 500 kDa γPGA > Pluronic F68 > control. Using EPO-dependent F-36E cells, we found that the effect of additives on rEPO quality was 500 kDa γPGA > 50 kDa γPGA > control > Pluronic F68. γPGA has an enhancement effect on the quality of rEPO produced by CHO cells.

Original language	English
Pages (from-to)	350-354
Number of pages	5
Journal	Biotechnology and Biotechnological Equipment
Volume	28
Issue number	2
DOIs	https://doi.org/10.1080/13102818.2014.901675
Publication status	Published - 2014

Bibliographical note

Funding Information:
This study was supported by the Korea Sanhak Foundation, Republic of Korea.

Keywords

Additives
CHO cells
Chemically defined medium
EPO-dependent F-36E cells
Pluronic F68
Poly-γ-glutamic acid
Recombinant erythropoietin

ASJC Scopus subject areas

Biotechnology

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10.1080/13102818.2014.901675

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title = "Poly-γ-glutamic acid enhances the quality of recombant erythropoietin produced by CHO cells",

abstract = "The effect of poly-γ-glutamic acid (γPGA), which is produced by Bacillus sp., on the production of recombinant erythropoietin (rEPO) by Chinese hamster ovary (CHO) cells in suspension culture was evaluated. The growth, viability, and productivity of recombinant CHO cells were investigated in a chemically defined medium with 50 and 500 kD γPGAs at 0.075% or with Pluronic F68. Cell growth with the two γPGAs was lower than that with Pluronic F68 but significantly higher than that without any additive (control). The effect of additives on rEPO productivity was 50 kDa γPGA > 500 kDa γPGA > Pluronic F68 > control. Using EPO-dependent F-36E cells, we found that the effect of additives on rEPO quality was 500 kDa γPGA > 50 kDa γPGA > control > Pluronic F68. γPGA has an enhancement effect on the quality of rEPO produced by CHO cells.",

keywords = "Additives, CHO cells, Chemically defined medium, EPO-dependent F-36E cells, Pluronic F68, Poly-γ-glutamic acid, Recombinant erythropoietin",

author = "Kim, {Tae Gon} and Cho, {Young Chol} and Chun, {Bok Hwan} and Park, {Sung Hyo} and Lee, {Hoi Seon} and Namhyun Chung",

note = "Funding Information: This study was supported by the Korea Sanhak Foundation, Republic of Korea.",

year = "2014",

doi = "10.1080/13102818.2014.901675",

language = "English",

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pages = "350--354",

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AU - Kim, Tae Gon

AU - Cho, Young Chol

AU - Chun, Bok Hwan

AU - Park, Sung Hyo

AU - Lee, Hoi Seon

AU - Chung, Namhyun

N1 - Funding Information: This study was supported by the Korea Sanhak Foundation, Republic of Korea.

PY - 2014

Y1 - 2014

N2 - The effect of poly-γ-glutamic acid (γPGA), which is produced by Bacillus sp., on the production of recombinant erythropoietin (rEPO) by Chinese hamster ovary (CHO) cells in suspension culture was evaluated. The growth, viability, and productivity of recombinant CHO cells were investigated in a chemically defined medium with 50 and 500 kD γPGAs at 0.075% or with Pluronic F68. Cell growth with the two γPGAs was lower than that with Pluronic F68 but significantly higher than that without any additive (control). The effect of additives on rEPO productivity was 50 kDa γPGA > 500 kDa γPGA > Pluronic F68 > control. Using EPO-dependent F-36E cells, we found that the effect of additives on rEPO quality was 500 kDa γPGA > 50 kDa γPGA > control > Pluronic F68. γPGA has an enhancement effect on the quality of rEPO produced by CHO cells.

AB - The effect of poly-γ-glutamic acid (γPGA), which is produced by Bacillus sp., on the production of recombinant erythropoietin (rEPO) by Chinese hamster ovary (CHO) cells in suspension culture was evaluated. The growth, viability, and productivity of recombinant CHO cells were investigated in a chemically defined medium with 50 and 500 kD γPGAs at 0.075% or with Pluronic F68. Cell growth with the two γPGAs was lower than that with Pluronic F68 but significantly higher than that without any additive (control). The effect of additives on rEPO productivity was 50 kDa γPGA > 500 kDa γPGA > Pluronic F68 > control. Using EPO-dependent F-36E cells, we found that the effect of additives on rEPO quality was 500 kDa γPGA > 50 kDa γPGA > control > Pluronic F68. γPGA has an enhancement effect on the quality of rEPO produced by CHO cells.

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KW - CHO cells

KW - Chemically defined medium

KW - EPO-dependent F-36E cells

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KW - Poly-γ-glutamic acid

KW - Recombinant erythropoietin

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Poly-γ-glutamic acid enhances the quality of recombant erythropoietin produced by CHO cells

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Keywords

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