Poly-γ-glutamic acid suppresses osteoclastogenesis in human osteoclast precursors and prevents joint damage in a collagen-induced murine arthritis model

Bitnara Lee; Sungsin Jo; Sung Min Kim; Mi La Cho; Sung Hwan Park; Jeehee Youn; Jong Dae Ji; Tae Hwan Kim

doi:10.1016/j.imlet.2018.09.004

Poly-γ-glutamic acid suppresses osteoclastogenesis in human osteoclast precursors and prevents joint damage in a collagen-induced murine arthritis model

Bitnara Lee, Sungsin Jo, Sung Min Kim, Mi La Cho, Sung Hwan Park, Jeehee Youn, Jong Dae Ji, Tae Hwan Kim

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Poly–γ-glutamic acid (γ-PGA), a natural polymer derived from Bacillus subtilis, shows anti-inflammatory activity. However, the effects of γ-PGA on osteoclasts, which are important cells for joint destruction in inflammatory diseases such as rheumatoid arthritis (RA), have not yet been reported. In this study, we show that γ-PGA markedly inhibits osteoclast differentiation in normal PBMC-derived osteoclast precursors and in synovial fluid macrophages of patients with RA. γ-PGA also reduces RANK expression by down-regulating M-CSF receptors. Additionally, oral administration of γ-PGA attenuated bone destruction in a collagen-induced arthritis (CIA) model, demonstrating decreases in inflammation, cartilage damage, and osteoclast formation in histological analyses. Taken together, these data suggest that γ-PGA could be a good candidate for therapeutic prevention of joint destruction in RA.

Original language	English
Pages (from-to)	80-86
Number of pages	7
Journal	Immunology Letters
Volume	203
DOIs	https://doi.org/10.1016/j.imlet.2018.09.004
Publication status	Published - 2018 Nov

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology ( 2013R1A1A2009617 ) and the Ministry of Science, ICT, & Future ( 2016R1A2B4008606 ), and a grant of the Korea Health Technology R&D project through the Korea Health Industry Development Institute (KHIDI) , funded by the Ministry of Health & Welfare, Republic of Korea ( HI17C0888 and HI15C1062 ). We thank Dr. Dong Soo Han (Department of Internal Medicine, Hanyang University Guri Hospital, Guri, Korea) for providing experimental materials.

Publisher Copyright:
© 2018 European Federation of Immunological Societies

Keywords

Osteoclast
Poly-γ-glutamic acid (γ -PGA)
Rheumatoid arthritis

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.imlet.2018.09.004

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title = "Poly-γ-glutamic acid suppresses osteoclastogenesis in human osteoclast precursors and prevents joint damage in a collagen-induced murine arthritis model",

abstract = "Poly–γ-glutamic acid (γ-PGA), a natural polymer derived from Bacillus subtilis, shows anti-inflammatory activity. However, the effects of γ-PGA on osteoclasts, which are important cells for joint destruction in inflammatory diseases such as rheumatoid arthritis (RA), have not yet been reported. In this study, we show that γ-PGA markedly inhibits osteoclast differentiation in normal PBMC-derived osteoclast precursors and in synovial fluid macrophages of patients with RA. γ-PGA also reduces RANK expression by down-regulating M-CSF receptors. Additionally, oral administration of γ-PGA attenuated bone destruction in a collagen-induced arthritis (CIA) model, demonstrating decreases in inflammation, cartilage damage, and osteoclast formation in histological analyses. Taken together, these data suggest that γ-PGA could be a good candidate for therapeutic prevention of joint destruction in RA.",

keywords = "Osteoclast, Poly-γ-glutamic acid (γ -PGA), Rheumatoid arthritis",

author = "Bitnara Lee and Sungsin Jo and Kim, {Sung Min} and Cho, {Mi La} and Park, {Sung Hwan} and Jeehee Youn and Ji, {Jong Dae} and Kim, {Tae Hwan}",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology ( 2013R1A1A2009617 ) and the Ministry of Science, ICT, & Future ( 2016R1A2B4008606 ), and a grant of the Korea Health Technology R&D project through the Korea Health Industry Development Institute (KHIDI) , funded by the Ministry of Health & Welfare, Republic of Korea ( HI17C0888 and HI15C1062 ). We thank Dr. Dong Soo Han (Department of Internal Medicine, Hanyang University Guri Hospital, Guri, Korea) for providing experimental materials. Publisher Copyright: {\textcopyright} 2018 European Federation of Immunological Societies",

year = "2018",

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doi = "10.1016/j.imlet.2018.09.004",

language = "English",

volume = "203",

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AU - Jo, Sungsin

AU - Kim, Sung Min

AU - Cho, Mi La

AU - Park, Sung Hwan

AU - Youn, Jeehee

AU - Ji, Jong Dae

AU - Kim, Tae Hwan

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology ( 2013R1A1A2009617 ) and the Ministry of Science, ICT, & Future ( 2016R1A2B4008606 ), and a grant of the Korea Health Technology R&D project through the Korea Health Industry Development Institute (KHIDI) , funded by the Ministry of Health & Welfare, Republic of Korea ( HI17C0888 and HI15C1062 ). We thank Dr. Dong Soo Han (Department of Internal Medicine, Hanyang University Guri Hospital, Guri, Korea) for providing experimental materials. Publisher Copyright: © 2018 European Federation of Immunological Societies

PY - 2018/11

Y1 - 2018/11

N2 - Poly–γ-glutamic acid (γ-PGA), a natural polymer derived from Bacillus subtilis, shows anti-inflammatory activity. However, the effects of γ-PGA on osteoclasts, which are important cells for joint destruction in inflammatory diseases such as rheumatoid arthritis (RA), have not yet been reported. In this study, we show that γ-PGA markedly inhibits osteoclast differentiation in normal PBMC-derived osteoclast precursors and in synovial fluid macrophages of patients with RA. γ-PGA also reduces RANK expression by down-regulating M-CSF receptors. Additionally, oral administration of γ-PGA attenuated bone destruction in a collagen-induced arthritis (CIA) model, demonstrating decreases in inflammation, cartilage damage, and osteoclast formation in histological analyses. Taken together, these data suggest that γ-PGA could be a good candidate for therapeutic prevention of joint destruction in RA.

AB - Poly–γ-glutamic acid (γ-PGA), a natural polymer derived from Bacillus subtilis, shows anti-inflammatory activity. However, the effects of γ-PGA on osteoclasts, which are important cells for joint destruction in inflammatory diseases such as rheumatoid arthritis (RA), have not yet been reported. In this study, we show that γ-PGA markedly inhibits osteoclast differentiation in normal PBMC-derived osteoclast precursors and in synovial fluid macrophages of patients with RA. γ-PGA also reduces RANK expression by down-regulating M-CSF receptors. Additionally, oral administration of γ-PGA attenuated bone destruction in a collagen-induced arthritis (CIA) model, demonstrating decreases in inflammation, cartilage damage, and osteoclast formation in histological analyses. Taken together, these data suggest that γ-PGA could be a good candidate for therapeutic prevention of joint destruction in RA.

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Poly-γ-glutamic acid suppresses osteoclastogenesis in human osteoclast precursors and prevents joint damage in a collagen-induced murine arthritis model

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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