Abstract
Acetate is regarded as a sustainable microbial feedstock that is synthesized from biowastes such as synthesis gas (syngas), carbon dioxide, lignocellulose, or organic waste. In this study, Methylorubrum extorquens AM1 was engineered to improve the production of bioplastic poly-3-hydroxybutyrate (PHB) using acetate as the sole carbon source. To utilize acetate as a carbon source and methanol as an energy source, acs encoding acetyl-CoA synthetase and fdh from Burkholderia stabilis were overexpressed, while ftfL involved in the assimilation of methanol into formyl-tetrahydrofolate was deleted. The yields of biomass and PHB from acetate significantly improved, and the growth rate and PHB content of the bacteria increased. In addition, sustainability of the PHB production was demonstrated using acetate derived from carbon dioxide and syngas. This study shows that biopolymers could be synthesized efficiently using acetate as the sole carbon source through metabolic engineering and the supply of energy cofactors.
Original language | English |
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Article number | 127127 |
Journal | Bioresource technology |
Volume | 353 |
DOIs | |
Publication status | Published - 2022 Jun |
Bibliographical note
Funding Information:This research was supported by C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2015M3D3A1A01064919 to M.-K.O. and 2018M3D3A1A01055733 to B.-K.C.).
Publisher Copyright:
© 2022 Elsevier Ltd
Keywords
- Acetate
- C1 gas
- Metabolic engineering
- Methylorubrum extorquens AM1
- Poly-3-hydroxybutyrate
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal