TY - JOUR
T1 - Poly-3-hydroxybutyrate production in acetate minimal medium using engineered Methylorubrum extorquens AM1
AU - Yoon, Jihee
AU - Bae, Jiyun
AU - Kang, Seulgi
AU - Cho, Byung Kwan
AU - Oh, Min Kyu
N1 - Funding Information:
This research was supported by C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2015M3D3A1A01064919 to M.-K.O. and 2018M3D3A1A01055733 to B.-K.C.).
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/6
Y1 - 2022/6
N2 - Acetate is regarded as a sustainable microbial feedstock that is synthesized from biowastes such as synthesis gas (syngas), carbon dioxide, lignocellulose, or organic waste. In this study, Methylorubrum extorquens AM1 was engineered to improve the production of bioplastic poly-3-hydroxybutyrate (PHB) using acetate as the sole carbon source. To utilize acetate as a carbon source and methanol as an energy source, acs encoding acetyl-CoA synthetase and fdh from Burkholderia stabilis were overexpressed, while ftfL involved in the assimilation of methanol into formyl-tetrahydrofolate was deleted. The yields of biomass and PHB from acetate significantly improved, and the growth rate and PHB content of the bacteria increased. In addition, sustainability of the PHB production was demonstrated using acetate derived from carbon dioxide and syngas. This study shows that biopolymers could be synthesized efficiently using acetate as the sole carbon source through metabolic engineering and the supply of energy cofactors.
AB - Acetate is regarded as a sustainable microbial feedstock that is synthesized from biowastes such as synthesis gas (syngas), carbon dioxide, lignocellulose, or organic waste. In this study, Methylorubrum extorquens AM1 was engineered to improve the production of bioplastic poly-3-hydroxybutyrate (PHB) using acetate as the sole carbon source. To utilize acetate as a carbon source and methanol as an energy source, acs encoding acetyl-CoA synthetase and fdh from Burkholderia stabilis were overexpressed, while ftfL involved in the assimilation of methanol into formyl-tetrahydrofolate was deleted. The yields of biomass and PHB from acetate significantly improved, and the growth rate and PHB content of the bacteria increased. In addition, sustainability of the PHB production was demonstrated using acetate derived from carbon dioxide and syngas. This study shows that biopolymers could be synthesized efficiently using acetate as the sole carbon source through metabolic engineering and the supply of energy cofactors.
KW - Acetate
KW - C1 gas
KW - Metabolic engineering
KW - Methylorubrum extorquens AM1
KW - Poly-3-hydroxybutyrate
UR - http://www.scopus.com/inward/record.url?scp=85128226697&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2022.127127
DO - 10.1016/j.biortech.2022.127127
M3 - Article
C2 - 35398538
AN - SCOPUS:85128226697
SN - 0960-8524
VL - 353
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 127127
ER -
