Polypyrimidine tract-binding protein interacts with HnRNP L

Bumsuk Hahm; Ook H. Cho; Jung E. Kim; Yoon K. Kim; Jong H. Kim; Young L. Oh; Sung K. Jang

doi:10.1016/S0014-5793(98)00269-5

Polypyrimidine tract-binding protein interacts with HnRNP L

Bumsuk Hahm, Ook H. Cho, Jung E. Kim, Yoon K. Kim, Jong H. Kim, Young L. Oh, Sung K. Jang

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

Polypyrimidine tract-binding protein (PTB) is involved in pre-mRNA splicing and internal ribosomal entry site (IRES)-dependent translation. In order to identify cellular protein(s) interacting with PTB, we performed a yeast two-hybrid screening. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) was identified as a PTB-binding protein. The interaction between PTB and hnRNP L was confirmed in an in vitro binding assay. Both PTB and hnRNP L were found to localize in the nucleoplasm, excepting the nucleoli, in HeLa cells by the green fluorescent protein (GFP)-fused protein detection method. The N-terminal half of PTB (aa 1-329) and most of hnRNP L (aa 141-558) is required for the interaction between PTB and hnRNP L.

Original language	English
Pages (from-to)	401-406
Number of pages	6
Journal	FEBS Letters
Volume	425
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00269-5
Publication status	Published - 1998 Apr 3
Externally published	Yes

Keywords

Heterogenous nuclear ribonucleoprotein L
Polypyrimidine tract-binding protein
Protein-protein interaction

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(98)00269-5

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title = "Polypyrimidine tract-binding protein interacts with HnRNP L",

abstract = "Polypyrimidine tract-binding protein (PTB) is involved in pre-mRNA splicing and internal ribosomal entry site (IRES)-dependent translation. In order to identify cellular protein(s) interacting with PTB, we performed a yeast two-hybrid screening. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) was identified as a PTB-binding protein. The interaction between PTB and hnRNP L was confirmed in an in vitro binding assay. Both PTB and hnRNP L were found to localize in the nucleoplasm, excepting the nucleoli, in HeLa cells by the green fluorescent protein (GFP)-fused protein detection method. The N-terminal half of PTB (aa 1-329) and most of hnRNP L (aa 141-558) is required for the interaction between PTB and hnRNP L.",

keywords = "Heterogenous nuclear ribonucleoprotein L, Polypyrimidine tract-binding protein, Protein-protein interaction",

author = "Bumsuk Hahm and Cho, {Ook H.} and Kim, {Jung E.} and Kim, {Yoon K.} and Kim, {Jong H.} and Oh, {Young L.} and Jang, {Sung K.}",

note = "Funding Information: We are indebted to Dr. Maurice Swanson (University of Florida, Gainesville, FL) for kindly providing plasmid pHCL3. The present study was supported in part by a grant from the G7 program, the Ministry of Science and Technology, KOESEF BSRI-97-4434, KOESEF through SRC for cell differentiation, and the POSTECH/BSRI special fund.",

year = "1998",

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doi = "10.1016/S0014-5793(98)00269-5",

language = "English",

volume = "425",

pages = "401--406",

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T1 - Polypyrimidine tract-binding protein interacts with HnRNP L

AU - Hahm, Bumsuk

AU - Cho, Ook H.

AU - Kim, Jung E.

AU - Kim, Yoon K.

AU - Kim, Jong H.

AU - Oh, Young L.

AU - Jang, Sung K.

N1 - Funding Information: We are indebted to Dr. Maurice Swanson (University of Florida, Gainesville, FL) for kindly providing plasmid pHCL3. The present study was supported in part by a grant from the G7 program, the Ministry of Science and Technology, KOESEF BSRI-97-4434, KOESEF through SRC for cell differentiation, and the POSTECH/BSRI special fund.

PY - 1998/4/3

Y1 - 1998/4/3

N2 - Polypyrimidine tract-binding protein (PTB) is involved in pre-mRNA splicing and internal ribosomal entry site (IRES)-dependent translation. In order to identify cellular protein(s) interacting with PTB, we performed a yeast two-hybrid screening. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) was identified as a PTB-binding protein. The interaction between PTB and hnRNP L was confirmed in an in vitro binding assay. Both PTB and hnRNP L were found to localize in the nucleoplasm, excepting the nucleoli, in HeLa cells by the green fluorescent protein (GFP)-fused protein detection method. The N-terminal half of PTB (aa 1-329) and most of hnRNP L (aa 141-558) is required for the interaction between PTB and hnRNP L.

AB - Polypyrimidine tract-binding protein (PTB) is involved in pre-mRNA splicing and internal ribosomal entry site (IRES)-dependent translation. In order to identify cellular protein(s) interacting with PTB, we performed a yeast two-hybrid screening. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) was identified as a PTB-binding protein. The interaction between PTB and hnRNP L was confirmed in an in vitro binding assay. Both PTB and hnRNP L were found to localize in the nucleoplasm, excepting the nucleoli, in HeLa cells by the green fluorescent protein (GFP)-fused protein detection method. The N-terminal half of PTB (aa 1-329) and most of hnRNP L (aa 141-558) is required for the interaction between PTB and hnRNP L.

KW - Heterogenous nuclear ribonucleoprotein L

KW - Polypyrimidine tract-binding protein

KW - Protein-protein interaction

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DO - 10.1016/S0014-5793(98)00269-5

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AN - SCOPUS:0032478588

SN - 0014-5793

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EP - 406

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Polypyrimidine tract-binding protein interacts with HnRNP L

Abstract

Keywords

ASJC Scopus subject areas

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