Porcine testicular extract inhibits T cell proliferation by blocking cell cycle transition from G 1 phase to S phase

Han Hyoung Lee; Suk Jun Lee; Sunhee Kim; Seonah Jeong; Manheum Na; Dong Mok Lee; Yong Pil Cheon; Ki Ho Lee; Inho Choi; Taehoon Chun

doi:10.1007/s10529-012-0907-6

Porcine testicular extract inhibits T cell proliferation by blocking cell cycle transition from G ₁ phase to S phase

Han Hyoung Lee, Suk Jun Lee, Sunhee Kim, Seonah Jeong, Manheum Na, Dong Mok Lee, Yong Pil Cheon, Ki Ho Lee, Inho Choi, Taehoon Chun

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have examined the effects of the porcine testicular extract on T cell activation: proliferation and secretion of cytokines (IL-2 and IFN-γ) by activated T cells were severely decreased after treatment with porcine testicular extract. The extract produced an immunosuppressive effect and inhibited the proliferation of activated T cells by blocking the cell cycle transition from the G ₁ phase to S phase. These effects were mediated by a decrease in the expression of cyclin D1 and cyclin E and constitutive expression of p27 ^KIP1 after T cell activation.

Original language	English
Pages (from-to)	1225-1233
Number of pages	9
Journal	Biotechnology letters
Volume	34
Issue number	7
DOIs	https://doi.org/10.1007/s10529-012-0907-6
Publication status	Published - 2012 Jul

Bibliographical note

Funding Information:
Acknowledgments This study was supported by Technology Development Program for Agriculture and Forestry (project number: 108089-3), Ministry for Agriculture, Forestry and Fisheries, Republic of Korea and Next-Generation BioGreen 21 program (No. PJ008089), Rural Development Administration, Republic of Korea.

Keywords

Cell cycle
Immunosuppressant
Porcine testicular extract
Steroid sex hormone
T cell activity

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1007/s10529-012-0907-6

Cite this

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title = "Porcine testicular extract inhibits T cell proliferation by blocking cell cycle transition from G 1 phase to S phase",

abstract = "Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have examined the effects of the porcine testicular extract on T cell activation: proliferation and secretion of cytokines (IL-2 and IFN-γ) by activated T cells were severely decreased after treatment with porcine testicular extract. The extract produced an immunosuppressive effect and inhibited the proliferation of activated T cells by blocking the cell cycle transition from the G 1 phase to S phase. These effects were mediated by a decrease in the expression of cyclin D1 and cyclin E and constitutive expression of p27 KIP1 after T cell activation.",

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note = "Funding Information: Acknowledgments This study was supported by Technology Development Program for Agriculture and Forestry (project number: 108089-3), Ministry for Agriculture, Forestry and Fisheries, Republic of Korea and Next-Generation BioGreen 21 program (No. PJ008089), Rural Development Administration, Republic of Korea.",

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AU - Lee, Han Hyoung

AU - Lee, Suk Jun

AU - Kim, Sunhee

AU - Jeong, Seonah

AU - Na, Manheum

AU - Lee, Dong Mok

AU - Cheon, Yong Pil

AU - Lee, Ki Ho

AU - Choi, Inho

AU - Chun, Taehoon

N1 - Funding Information: Acknowledgments This study was supported by Technology Development Program for Agriculture and Forestry (project number: 108089-3), Ministry for Agriculture, Forestry and Fisheries, Republic of Korea and Next-Generation BioGreen 21 program (No. PJ008089), Rural Development Administration, Republic of Korea.

PY - 2012/7

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N2 - Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have examined the effects of the porcine testicular extract on T cell activation: proliferation and secretion of cytokines (IL-2 and IFN-γ) by activated T cells were severely decreased after treatment with porcine testicular extract. The extract produced an immunosuppressive effect and inhibited the proliferation of activated T cells by blocking the cell cycle transition from the G 1 phase to S phase. These effects were mediated by a decrease in the expression of cyclin D1 and cyclin E and constitutive expression of p27 KIP1 after T cell activation.

AB - Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have examined the effects of the porcine testicular extract on T cell activation: proliferation and secretion of cytokines (IL-2 and IFN-γ) by activated T cells were severely decreased after treatment with porcine testicular extract. The extract produced an immunosuppressive effect and inhibited the proliferation of activated T cells by blocking the cell cycle transition from the G 1 phase to S phase. These effects were mediated by a decrease in the expression of cyclin D1 and cyclin E and constitutive expression of p27 KIP1 after T cell activation.

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KW - Steroid sex hormone

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