Porous gelatin-siloxane hybrid scaffolds with biomimetic structure and properties for bone tissue regeneration

Bo Lei, Kwan Ha Shin, Young Hag Koh, Hyoun Ee Kim

    Research output: Contribution to journalArticlepeer-review

    27 Citations (Scopus)

    Abstract

    We produced highly porous gelatin-siloxane (GLAS) hybrid scaffolds with biomimetic anisotropic porous structure, physiochemical properties, mechanical behaviors and biological functions by treating gelatin-siloxane hybrid gels in an ammonium hydroxide solution. The siloxane used as an inorganic phase could effectively crosslink the gelatin polymer, which allowed for the unidirectional enlargement of ammonia vacuoles during ammonium hydroxide treatment. This created aligned pores in an axial direction when the siloxane contents (10 and 20 wt %) were high. In addition, the gelatin polymer could be uniformly hybridized with the siloxane phase at the molecular level, while intense interaction between these two phases could be achieved. This resulted in a significant increase in mechanical properties. The GLA-S hybrid scaffold with a siloxane content of 10 wt % showed reasonably high compressive yield strength of 4.260.1 MPa and compressive modulus of 8465 MPa at a porosity of 86 vol %, which would be comparable to those of natural cancellous bone. In addition, the GLA-S hybrid scaffold had good biocompatibility assessed by in vitro cell tests using pre-osteoblast MC3T3-E1 cells.

    Original languageEnglish
    Pages (from-to)1528-1536
    Number of pages9
    JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
    Volume102
    Issue number7
    DOIs
    Publication statusPublished - 2014 Oct 1

    Bibliographical note

    Publisher Copyright:
    ©2014 Wiley Periodicals, Inc.

    Keywords

    • Gelatin
    • Porous structure
    • Scaffold
    • Silica
    • Tissue regeneration

    ASJC Scopus subject areas

    • Biomaterials
    • Biomedical Engineering

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