Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF

Mi Young Jeong, Eung Ryoung Lee, Cheol Won Yun, Ssang Goo Cho, Yong Jin Choi

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    XaiF, a novel 32 kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearothermophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3′-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3′-UTR of the mRNA.

    Original languageEnglish
    Pages (from-to)153-158
    Number of pages6
    JournalBiochemical and biophysical research communications
    Volume351
    Issue number1
    DOIs
    Publication statusPublished - 2006 Dec 8

    Bibliographical note

    Funding Information:
    This paper was supported by Konkuk University in 2004.

    Keywords

    • 3′-Untranslational region
    • Bacillus stearothermophilus No. 236
    • Post-transcriptional regulation
    • Xylanase
    • mRNA binding protein
    • mRNA stability
    • xaiF
    • xynA

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Fingerprint

    Dive into the research topics of 'Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF'. Together they form a unique fingerprint.

    Cite this