Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF

Mi Young Jeong; Eung Ryoung Lee; Cheol Won Yun; Ssang Goo Cho; Yong Jin Choi

doi:10.1016/j.bbrc.2006.10.009

Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF

Mi Young Jeong, Eung Ryoung Lee, Cheol Won Yun, Ssang Goo Cho, Yong Jin Choi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

XaiF, a novel 32 kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearothermophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3′-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3′-UTR of the mRNA.

Original language	English
Pages (from-to)	153-158
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	351
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2006.10.009
Publication status	Published - 2006 Dec 8

Bibliographical note

Funding Information:
This paper was supported by Konkuk University in 2004.

Keywords

3′-Untranslational region
Bacillus stearothermophilus No. 236
Post-transcriptional regulation
Xylanase
mRNA binding protein
mRNA stability
xaiF
xynA

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2006.10.009

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title = "Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF",

abstract = "XaiF, a novel 32 kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearothermophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3′-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3′-UTR of the mRNA.",

keywords = "3′-Untranslational region, Bacillus stearothermophilus No. 236, Post-transcriptional regulation, Xylanase, mRNA binding protein, mRNA stability, xaiF, xynA",

author = "Jeong, {Mi Young} and Lee, {Eung Ryoung} and Yun, {Cheol Won} and Cho, {Ssang Goo} and Choi, {Yong Jin}",

note = "Funding Information: This paper was supported by Konkuk University in 2004.",

year = "2006",

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doi = "10.1016/j.bbrc.2006.10.009",

language = "English",

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T1 - Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF

AU - Jeong, Mi Young

AU - Lee, Eung Ryoung

AU - Yun, Cheol Won

AU - Cho, Ssang Goo

AU - Choi, Yong Jin

N1 - Funding Information: This paper was supported by Konkuk University in 2004.

PY - 2006/12/8

Y1 - 2006/12/8

N2 - XaiF, a novel 32 kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearothermophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3′-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3′-UTR of the mRNA.

AB - XaiF, a novel 32 kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearothermophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3′-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3′-UTR of the mRNA.

KW - 3′-Untranslational region

KW - Bacillus stearothermophilus No. 236

KW - Post-transcriptional regulation

KW - Xylanase

KW - mRNA binding protein

KW - mRNA stability

KW - xaiF

KW - xynA

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U2 - 10.1016/j.bbrc.2006.10.009

DO - 10.1016/j.bbrc.2006.10.009

M3 - Article

C2 - 17055461

AN - SCOPUS:33750287572

SN - 0006-291X

VL - 351

SP - 153

EP - 158

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

IS - 1

ER -

Post-transcriptional regulation of the xynA expression by a novel mRNA binding protein, XaiF

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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