XaiF, a novel 32 kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearothermophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3′-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3′-UTR of the mRNA.
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 2006 Dec 8|
Bibliographical noteFunding Information:
This paper was supported by Konkuk University in 2004.
- 3′-Untranslational region
- Bacillus stearothermophilus No. 236
- Post-transcriptional regulation
- mRNA binding protein
- mRNA stability
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology