Abstract
XaiF, a novel 32 kDa protein encoded by the ORF located in the immediate downstream of the xynA gene of Bacillus stearothermophilus No. 236, was identified to be the xylanase-specific trans-activator. In this study, the positive effect of XaiF was confirmed to be xylanase-specific, and the results from Northern blot and in vitro transcription assays showed that the XaiF increased the xynA transcripts at post-transcriptional step. Moreover, analysis of the mRNA decay rate led to the assertion that the XaiF functions to stabilize the xynA mRNA. Intriguingly, in vitro RNA-protein binding assay and analysis using gst-xynA 3′-UTR chimeric gene constructs demonstrated that the XaiF stabilizes xynA mRNA by direct binding onto the 3′-UTR of the mRNA.
Original language | English |
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Pages (from-to) | 153-158 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 351 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2006 Dec 8 |
Bibliographical note
Funding Information:This paper was supported by Konkuk University in 2004.
Keywords
- 3′-Untranslational region
- Bacillus stearothermophilus No. 236
- Post-transcriptional regulation
- Xylanase
- mRNA binding protein
- mRNA stability
- xaiF
- xynA
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology