Post-translational regulation of short vegetative phase as a major mechanism for thermoregulation of flowering

Jeong Hwan Lee, Kyung Sook Chung, Soon Kap Kim, Ji H oon Ahn

    Research output: Contribution to journalArticlepeer-review


    In contrast to our extensive knowledge of vernalization, we know relatively little about the regulation of ambient temperature-responsive flowering. Recent reports revealed that flowering locus M (FLM) and short vegetative phase (SVP) regulate high ambient temperature-responsive flowering through two different mechanisms: degradation of SVP protein and formation of a non-functional SVP-FLM-δ complex. To investigate further the mechanism of thermoregulation of flowering, we performed real-time quantitative polymerase chain reaction (RT-qPCR) and in vitro pull-down assays. We found that FLM-β and FLM-δ transcripts show similar absolute levels at different temperatures. Also, His-SVP protein bound to the GST-FLM-β or -δ proteins with similar binding intensities. These results suggest that functional SVP-FLM-β and non-functional SVP-FLM-δ complexes form similarly at warmer temperatures, thus indicating that post-translational regulation of SVP functions as a major mechanism for thermoregulation in flowering.

    Original languageEnglish
    Pages (from-to)e28193
    JournalPlant signaling & behavior
    Issue number3
    Publication statusPublished - 2014


    • Alternative splicing
    • Ambient temperature
    • Ambient temperature-responsive flowering
    • FLM-β
    • FLM-δ
    • SVP
    • thermoregulation

    ASJC Scopus subject areas

    • Medicine(all)


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