Precise base editing without unintended indels in human cells and mouse primary myoblasts

Da Eun Yoon, Na Rae Kim, Soo Ji Park, Tae Yeong Jeong, Bokkee Eun, Yongcheol Cho, Soo Yeon Lim, Hyunji Lee, Je Kyoung Seong, Kyoungmi Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Base editors are powerful tools for making precise single-nucleotide changes in the genome. However, they can lead to unintended insertions and deletions at the target sites, which is a significant limitation for clinical applications. In this study, we aimed to eliminate unwanted indels at the target sites caused by various evolved base editors. Accordingly, we applied dead Cas9 instead of nickase Cas9 in the base editors to induce accurate substitutions without indels. Additionally, we tested the use of chromatin-modulating peptides in the base editors to improve nucleotide conversion efficiency. We found that using both dead Cas9 and chromatin-modulating peptides in base editing improved the nucleotide substitution efficiency without unintended indel mutations at the desired target sites in human cell lines and mouse primary myoblasts. Furthermore, the proposed scheme had fewer off-target effects than conventional base editors at the DNA level. These results indicate that the suggested approach is promising for the development of more accurate and safer base editing techniques for use in clinical applications.

Original languageEnglish
Pages (from-to)2586-2595
Number of pages10
JournalExperimental and Molecular Medicine
Volume55
Issue number12
DOIs
Publication statusPublished - 2023 Dec

Bibliographical note

Publisher Copyright:
© 2023, The Author(s).

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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