Pyrethroid insecticides have been effective and powerful for controlling mosquitoes. However, abuse of these insecticides increases the number of resistant mosquitoes. In this study, Culex pipiens pallens and Aedes koreicus were collected from an artificial reservoir in the vicinity of a populated area in Korea, which is also a migratory bird catchment area. To monitor resistance to pyrethroid insecticides in mosquitoes, genomic DNA from the collected mosquitoes was sequenced for the kdr mutation in the voltage-gated sodium channel (VGSC) gene. As a result, three samples with homozygous resistance (17.6%) and one with heterozygous resistance (5.9%) were found among 17 Cx. pipiens pallens specimens. One of the samples had a unique sequence at the amplified VGSC region. Of the 15 Ae. koreicus, no insecticide resistant individuals were found. In Korea, this is the first report of kdr genetic traits in Ae. koreicus and Cx. pipiens pallens and of a unique VGSC allele in Cx. pipiens pallens. Further investigation is needed to monitor the kdr resistance of these species in Korea and to determine how the unique sequence found in Cx. pipiens pallens is related to insecticide resistance.
Bibliographical noteFunding Information:
This research was supported by the Korea Environmental Industry & Technology Institute (KEITI). Project number: 2016000210003, Project name: Field application of biological mosquito management and impact assessment, and Government-wide R&D Fund project for infectious disease research (GFID), Republic of Korea (grant number: HG18C0046).
© 2019 The Entomological Society of Korea and John Wiley & Sons Australia, Ltd
- Aedes koreicus
- Culex pipiens pallens
- insecticide resistance
ASJC Scopus subject areas
- Insect Science