Preparation and biocompatibility of chitosan microcarriers as biomaterial

Xi Guang Chen, Cheng Sheng Liu, Chen Guang Liu, Xiang Hong Meng, Chong M. Lee, Hyun Jin Park

Research output: Contribution to journalArticlepeer-review

80 Citations (Scopus)


Chitosan microcarriers (100-200 μm) were prepared by the methods of emulsification and ethanol coagulant. It has smooth surface and was stable in phosphate buffer solution (PBS) of at pH 7.2 in the treatment of temperature 120°C and pressure 150 kPa. The chitosan microcarriers showed molecular affinity to the bovine serum proteins at pH 7.2. The adsorptive capacity of the microcarriers to the serum albumin was 6.8 mg protein/g chitosan bead. The chitosan microcarriers were found to have good biocompatibility and no cytotoxicity to both human and mouse fibroblasts in tissue cell culture. The fibroblasts well adhered on the smooth surface of the chitosan microcarriers and grew in high cell density. The results suggest a good potential of the chitosan microcarriers as a wound-healing biomaterial.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalBiochemical Engineering Journal
Issue number3
Publication statusPublished - 2006 Jan


  • Adsorption
  • Affinity
  • Microcarrier
  • Tissue cell culture

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering


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