Preparation and biocompatibility of chitosan microcarriers as biomaterial

Xi Guang Chen; Cheng Sheng Liu; Chen Guang Liu; Xiang Hong Meng; Chong M. Lee; Hyun Jin Park

doi:10.1016/j.bej.2005.08.021

Preparation and biocompatibility of chitosan microcarriers as biomaterial

Xi Guang Chen, Cheng Sheng Liu, Chen Guang Liu, Xiang Hong Meng, Chong M. Lee, Hyun Jin Park

Research output: Contribution to journal › Article › peer-review

87 Citations (Scopus)

Abstract

Chitosan microcarriers (100-200 μm) were prepared by the methods of emulsification and ethanol coagulant. It has smooth surface and was stable in phosphate buffer solution (PBS) of at pH 7.2 in the treatment of temperature 120°C and pressure 150 kPa. The chitosan microcarriers showed molecular affinity to the bovine serum proteins at pH 7.2. The adsorptive capacity of the microcarriers to the serum albumin was 6.8 mg protein/g chitosan bead. The chitosan microcarriers were found to have good biocompatibility and no cytotoxicity to both human and mouse fibroblasts in tissue cell culture. The fibroblasts well adhered on the smooth surface of the chitosan microcarriers and grew in high cell density. The results suggest a good potential of the chitosan microcarriers as a wound-healing biomaterial.

Original language	English
Pages (from-to)	269-274
Number of pages	6
Journal	Biochemical Engineering Journal
Volume	27
Issue number	3
DOIs	https://doi.org/10.1016/j.bej.2005.08.021
Publication status	Published - 2006 Jan

Bibliographical note

Funding Information:
We acknowledge the National Natural Science Foundation of China (30370344) and the Ministry of Education of China (2003-406) for financial support.

Keywords

Adsorption
Affinity
Microcarrier
Tissue cell culture

ASJC Scopus subject areas

Biotechnology
Environmental Engineering
Bioengineering
Biomedical Engineering

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10.1016/j.bej.2005.08.021

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title = "Preparation and biocompatibility of chitosan microcarriers as biomaterial",

abstract = "Chitosan microcarriers (100-200 μm) were prepared by the methods of emulsification and ethanol coagulant. It has smooth surface and was stable in phosphate buffer solution (PBS) of at pH 7.2 in the treatment of temperature 120°C and pressure 150 kPa. The chitosan microcarriers showed molecular affinity to the bovine serum proteins at pH 7.2. The adsorptive capacity of the microcarriers to the serum albumin was 6.8 mg protein/g chitosan bead. The chitosan microcarriers were found to have good biocompatibility and no cytotoxicity to both human and mouse fibroblasts in tissue cell culture. The fibroblasts well adhered on the smooth surface of the chitosan microcarriers and grew in high cell density. The results suggest a good potential of the chitosan microcarriers as a wound-healing biomaterial.",

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author = "Chen, {Xi Guang} and Liu, {Cheng Sheng} and Liu, {Chen Guang} and Meng, {Xiang Hong} and Lee, {Chong M.} and Park, {Hyun Jin}",

note = "Funding Information: We acknowledge the National Natural Science Foundation of China (30370344) and the Ministry of Education of China (2003-406) for financial support.",

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AU - Chen, Xi Guang

AU - Liu, Cheng Sheng

AU - Liu, Chen Guang

AU - Meng, Xiang Hong

AU - Lee, Chong M.

AU - Park, Hyun Jin

N1 - Funding Information: We acknowledge the National Natural Science Foundation of China (30370344) and the Ministry of Education of China (2003-406) for financial support.

PY - 2006/1

Y1 - 2006/1

N2 - Chitosan microcarriers (100-200 μm) were prepared by the methods of emulsification and ethanol coagulant. It has smooth surface and was stable in phosphate buffer solution (PBS) of at pH 7.2 in the treatment of temperature 120°C and pressure 150 kPa. The chitosan microcarriers showed molecular affinity to the bovine serum proteins at pH 7.2. The adsorptive capacity of the microcarriers to the serum albumin was 6.8 mg protein/g chitosan bead. The chitosan microcarriers were found to have good biocompatibility and no cytotoxicity to both human and mouse fibroblasts in tissue cell culture. The fibroblasts well adhered on the smooth surface of the chitosan microcarriers and grew in high cell density. The results suggest a good potential of the chitosan microcarriers as a wound-healing biomaterial.

AB - Chitosan microcarriers (100-200 μm) were prepared by the methods of emulsification and ethanol coagulant. It has smooth surface and was stable in phosphate buffer solution (PBS) of at pH 7.2 in the treatment of temperature 120°C and pressure 150 kPa. The chitosan microcarriers showed molecular affinity to the bovine serum proteins at pH 7.2. The adsorptive capacity of the microcarriers to the serum albumin was 6.8 mg protein/g chitosan bead. The chitosan microcarriers were found to have good biocompatibility and no cytotoxicity to both human and mouse fibroblasts in tissue cell culture. The fibroblasts well adhered on the smooth surface of the chitosan microcarriers and grew in high cell density. The results suggest a good potential of the chitosan microcarriers as a wound-healing biomaterial.

KW - Adsorption

KW - Affinity

KW - Microcarrier

KW - Tissue cell culture

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DO - 10.1016/j.bej.2005.08.021

M3 - Article

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SN - 1369-703X

VL - 27

SP - 269

EP - 274

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

IS - 3

ER -

Preparation and biocompatibility of chitosan microcarriers as biomaterial

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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