Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers

Ya Liu; Xiao Jie Cheng; Qi Feng Dang; Fang Kui Ma; Xi Guang Chen; Hyun Jin Park; Bum Keun Kim

doi:10.1007/s10856-011-4470-9

Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers

Ya Liu, Xiao Jie Cheng, Qi Feng Dang, Fang Kui Ma, Xi Guang Chen, Hyun Jin Park, Bum Keun Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by selfassembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS- ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P<0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.

Original language	English
Pages (from-to)	375-384
Number of pages	10
Journal	Journal of Materials Science: Materials in Medicine
Volume	23
Issue number	2
DOIs	https://doi.org/10.1007/s10856-011-4470-9
Publication status	Published - 2012 Feb

ASJC Scopus subject areas

Biophysics
Bioengineering
Biomaterials
Biomedical Engineering

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title = "Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers",

abstract = "Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by selfassembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS- ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P<0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.",

author = "Ya Liu and Cheng, {Xiao Jie} and Dang, {Qi Feng} and Ma, {Fang Kui} and Chen, {Xi Guang} and Park, {Hyun Jin} and Kim, {Bum Keun}",

note = "Funding Information: Acknowledgments This work was supported by grants from the National Natural Science Foundation of China (NSFC, 81071274), International S & T Cooperation Program of China (ISTCP, S2011GR0092).",

year = "2012",

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doi = "10.1007/s10856-011-4470-9",

language = "English",

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T1 - Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers

AU - Liu, Ya

AU - Cheng, Xiao Jie

AU - Dang, Qi Feng

AU - Ma, Fang Kui

AU - Chen, Xi Guang

AU - Park, Hyun Jin

AU - Kim, Bum Keun

N1 - Funding Information: Acknowledgments This work was supported by grants from the National Natural Science Foundation of China (NSFC, 81071274), International S & T Cooperation Program of China (ISTCP, S2011GR0092).

PY - 2012/2

Y1 - 2012/2

N2 - Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by selfassembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS- ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P<0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.

AB - Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by selfassembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS- ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P<0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.

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Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers

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