Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers

Ya Liu, Xiao Jie Cheng, Qi Feng Dang, Fang Kui Ma, Xi Guang Chen, Hyun Jin Park, Bum Keun Kim

    Research output: Contribution to journalArticlepeer-review

    34 Citations (Scopus)

    Abstract

    Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by selfassembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS- ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P<0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.

    Original languageEnglish
    Pages (from-to)375-384
    Number of pages10
    JournalJournal of Materials Science: Materials in Medicine
    Volume23
    Issue number2
    DOIs
    Publication statusPublished - 2012 Feb

    Bibliographical note

    Funding Information:
    Acknowledgments This work was supported by grants from the National Natural Science Foundation of China (NSFC, 81071274), International S & T Cooperation Program of China (ISTCP, S2011GR0092).

    ASJC Scopus subject areas

    • Biophysics
    • Bioengineering
    • Biomaterials
    • Biomedical Engineering

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