Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers

Ya Liu, Xiao Jie Cheng, Qi Feng Dang, Fang Kui Ma, Xi Guang Chen, Hyun Jin Park, Bum Keun Kim

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by selfassembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS- ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P<0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.

Original languageEnglish
Pages (from-to)375-384
Number of pages10
JournalJournal of Materials Science: Materials in Medicine
Issue number2
Publication statusPublished - 2012 Feb

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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