Preparation of γ-linolenic acid rich triacylglycerol from borage oil via a two-step lipase-catalyzed reaction

Hui Su Yoon, Yu Jin Lee, Byung Hee Kim, Yangha Kim, In Hwan Kim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

γ-Linolenic acid (GLA) rich triacylglycerol (TAG) was successfully synthesized from glyceride, instead of glycerol, and fatty acid (FA) via Lipozyme TL IM-catalyzed esterification as a novel strategy. In the first step, GLA was enriched into glyceride fraction from borage oil by Candida rugosa lipase-catalyzed hydrolysis. The glyceride was separated from the reaction mixture by molecular distillation. GLA was enriched from 20.64% in borage oil to 45.94% in the glyceride fraction under optimum conditions. In the second step, the Lipozyme TL IM-catalyzed synthesis of TAG was carried out with the glyceride, and the FA obtained by saponification of a portion of the glyceride. The optimum conditions were the temperature of 50°C, the enzyme loading of 10%, and the vacuum level of 20 mmHg, respectively. The maximum TAG content of approximately 92% was achieved after 12 h under the optimum conditions.

Original languageEnglish
Pages (from-to)215-224
Number of pages10
JournalJAOCS, Journal of the American Oil Chemists' Society
Volume100
Issue number3
DOIs
Publication statusPublished - 2023 Mar

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education NRF-2021R1A2C1003128.

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education NRF‐2021R1A2C1003128.

Funding Information:
National Research Foundation of Korea, Grant/Award Number: NRF‐2021R1A2C1003128 Funding information

Publisher Copyright:
© 2023 AOCS.

Keywords

  • Candida rugosa lipase
  • borage oil
  • enrichment
  • glyceride
  • triglyceride (TAG)
  • γ-linolenic acid (GLA)

ASJC Scopus subject areas

  • General Chemical Engineering
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'Preparation of γ-linolenic acid rich triacylglycerol from borage oil via a two-step lipase-catalyzed reaction'. Together they form a unique fingerprint.

Cite this