Preparation of high purity docosahexaenoic acid from microalgae oil in a packed bed reactor via two-step lipase-catalysed esterification

Eun Ju Lee, Moon Won Lee, Da Som No, Hee Jin Kim, Se Wook Oh, Yangha Kim, In Hwan Kim

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Highly pure docosahexaenoic acid (DHA) was produced successfully in a packed bed reactor via the two-step lipase-catalysed esterification using the fatty acid from microalgae (from Crypthecodinium cohnii) and ethanol as substrates. Lipozyme RM IM from Rhizomucor miehei was employed as a biocatalyst. In the first step, a temperature of 40 °C, a molar ratio of 1:4 (fatty acid to ethanol), and a water content of 0.6 wt% (based on the total substrate weight) were selected as optimum conditions. A maximum DHA concentration of ca. 90 wt% was achieved in the fatty acid fraction with an 89 wt% yield after 15 min. In the second step, the fatty acids from the first step were used as the substrate and a recursive reaction was conducted using identical optimized conditions. Following this second step, a DHA concentration of 100 wt% was achieved in conjunction with an 83.2 wt% yield after 25 min.

Original languageEnglish
Pages (from-to)330-337
Number of pages8
JournalJournal of Functional Foods
Volume21
DOIs
Publication statusPublished - 2016 Mar 1

Bibliographical note

Funding Information:
This research was supported by the project titled ‘Development of rapid detection system for foodborne pathogens to strengthen the food safety and to promote the sea foods consumption funded by the Ministry of Oceans and Fisheries (Project No. 20130281 ) Korea.

Keywords

  • Crypthecodinium cohnii
  • Docosahexaenoic acid (DHA)
  • Esterification
  • Lipase
  • Microalgae
  • Rhizomucor miehei

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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