Preparation of highly purified pinolenic acid from pine nut oil using a combination of enzymatic esterification and urea complexation

Da Som No; Ting Ting Zhao; Yangha Kim; Mi Ra Yoon; Jeom Sig Lee; In Hwan Kim

doi:10.1016/j.foodchem.2014.08.074

Preparation of highly purified pinolenic acid from pine nut oil using a combination of enzymatic esterification and urea complexation

Da Som No, Ting Ting Zhao, Yangha Kim, Mi Ra Yoon, Jeom Sig Lee, In Hwan Kim

Department of Public Health Sciences

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Pinolenic acid (PLA) is a polyunsaturated fatty acid of plant origin. PLA has been successfully enriched according to a two-step process involving lipase-catalysed esterification and urea complexation. For the first step, the fatty acids present in pine nut oil were selectively esterified with lauryl alcohol using Candida rugosa lipase. Under the optimum conditions of 0.1% enzyme loading, 10% additional water, and 15 °C, PLA was enriched up to 43 mol% from an initial value of 13 mol% in the pine nut oil. For the second step, the PLA-enriched fraction from the first step was subjected to a urea complexation process. In this way, PLA enrichments with purities greater than 95 mol% were obtained at urea to fatty acid ratios greater than 3:1 (wt/wt), and 100% pure PLA was produced at a urea to fatty acid ratio of 5:1 with an 8.7 mol% yield.

Original language	English
Pages (from-to)	386-393
Number of pages	8
Journal	Food Chemistry
Volume	170
DOIs	https://doi.org/10.1016/j.foodchem.2014.08.074
Publication status	Published - 2015 Mar 1

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science, and Technology ( 2013R1A1A2006050 ).

Publisher Copyright:
© 2014 Elsevier Ltd. All rights reserved.

Keywords

Candida rugosa lipase
Lipase-catalysed esterification
Pine nut oil
Pinolenic acid
Urea complexation

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2014.08.074

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title = "Preparation of highly purified pinolenic acid from pine nut oil using a combination of enzymatic esterification and urea complexation",

abstract = "Pinolenic acid (PLA) is a polyunsaturated fatty acid of plant origin. PLA has been successfully enriched according to a two-step process involving lipase-catalysed esterification and urea complexation. For the first step, the fatty acids present in pine nut oil were selectively esterified with lauryl alcohol using Candida rugosa lipase. Under the optimum conditions of 0.1% enzyme loading, 10% additional water, and 15 °C, PLA was enriched up to 43 mol% from an initial value of 13 mol% in the pine nut oil. For the second step, the PLA-enriched fraction from the first step was subjected to a urea complexation process. In this way, PLA enrichments with purities greater than 95 mol% were obtained at urea to fatty acid ratios greater than 3:1 (wt/wt), and 100% pure PLA was produced at a urea to fatty acid ratio of 5:1 with an 8.7 mol% yield.",

keywords = "Candida rugosa lipase, Lipase-catalysed esterification, Pine nut oil, Pinolenic acid, Urea complexation",

author = "No, {Da Som} and Zhao, {Ting Ting} and Yangha Kim and Yoon, {Mi Ra} and Lee, {Jeom Sig} and Kim, {In Hwan}",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science, and Technology ( 2013R1A1A2006050 ). Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",

year = "2015",

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doi = "10.1016/j.foodchem.2014.08.074",

language = "English",

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AU - No, Da Som

AU - Zhao, Ting Ting

AU - Kim, Yangha

AU - Yoon, Mi Ra

AU - Lee, Jeom Sig

AU - Kim, In Hwan

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science, and Technology ( 2013R1A1A2006050 ). Publisher Copyright: © 2014 Elsevier Ltd. All rights reserved.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Pinolenic acid (PLA) is a polyunsaturated fatty acid of plant origin. PLA has been successfully enriched according to a two-step process involving lipase-catalysed esterification and urea complexation. For the first step, the fatty acids present in pine nut oil were selectively esterified with lauryl alcohol using Candida rugosa lipase. Under the optimum conditions of 0.1% enzyme loading, 10% additional water, and 15 °C, PLA was enriched up to 43 mol% from an initial value of 13 mol% in the pine nut oil. For the second step, the PLA-enriched fraction from the first step was subjected to a urea complexation process. In this way, PLA enrichments with purities greater than 95 mol% were obtained at urea to fatty acid ratios greater than 3:1 (wt/wt), and 100% pure PLA was produced at a urea to fatty acid ratio of 5:1 with an 8.7 mol% yield.

AB - Pinolenic acid (PLA) is a polyunsaturated fatty acid of plant origin. PLA has been successfully enriched according to a two-step process involving lipase-catalysed esterification and urea complexation. For the first step, the fatty acids present in pine nut oil were selectively esterified with lauryl alcohol using Candida rugosa lipase. Under the optimum conditions of 0.1% enzyme loading, 10% additional water, and 15 °C, PLA was enriched up to 43 mol% from an initial value of 13 mol% in the pine nut oil. For the second step, the PLA-enriched fraction from the first step was subjected to a urea complexation process. In this way, PLA enrichments with purities greater than 95 mol% were obtained at urea to fatty acid ratios greater than 3:1 (wt/wt), and 100% pure PLA was produced at a urea to fatty acid ratio of 5:1 with an 8.7 mol% yield.

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KW - Urea complexation

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Preparation of highly purified pinolenic acid from pine nut oil using a combination of enzymatic esterification and urea complexation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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