Abstract
Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.
Original language | English |
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Pages (from-to) | 1965-1969 |
Number of pages | 5 |
Journal | Biotechnology letters |
Volume | 28 |
Issue number | 23 |
DOIs | |
Publication status | Published - 2006 Dec |
Bibliographical note
Funding Information:Acknowledgment This work was supported by research grant 2003-N-BI03-P-01 from the Korea Energy Management Corporation.
Keywords
- Immobilization
- Lipase
- Pretreatment
- Reuse
- Soybean oil
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology