Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity

Dong Hwan Lee; Jung Mo Kim; Seong Woo Kang; Ji Won Lee; Seung Wook Kim

doi:10.1007/s10529-006-9181-9

Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity

Dong Hwan Lee, Jung Mo Kim, Seong Woo Kang, Ji Won Lee, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.

Original language	English
Pages (from-to)	1965-1969
Number of pages	5
Journal	Biotechnology letters
Volume	28
Issue number	23
DOIs	https://doi.org/10.1007/s10529-006-9181-9
Publication status	Published - 2006 Dec

Keywords

Immobilization
Lipase
Pretreatment
Reuse
Soybean oil

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-006-9181-9

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title = "Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity",

abstract = "Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.",

keywords = "Immobilization, Lipase, Pretreatment, Reuse, Soybean oil",

author = "Lee, {Dong Hwan} and Kim, {Jung Mo} and Kang, {Seong Woo} and Lee, {Ji Won} and Kim, {Seung Wook}",

note = "Funding Information: Acknowledgment This work was supported by research grant 2003-N-BI03-P-01 from the Korea Energy Management Corporation.",

year = "2006",

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doi = "10.1007/s10529-006-9181-9",

language = "English",

volume = "28",

pages = "1965--1969",

journal = "Biotechnology Letters",

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TY - JOUR

T1 - Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity

AU - Lee, Dong Hwan

AU - Kim, Jung Mo

AU - Kang, Seong Woo

AU - Lee, Ji Won

AU - Kim, Seung Wook

N1 - Funding Information: Acknowledgment This work was supported by research grant 2003-N-BI03-P-01 from the Korea Energy Management Corporation.

PY - 2006/12

Y1 - 2006/12

N2 - Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.

AB - Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.

KW - Immobilization

KW - Lipase

KW - Pretreatment

KW - Reuse

KW - Soybean oil

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U2 - 10.1007/s10529-006-9181-9

DO - 10.1007/s10529-006-9181-9

M3 - Article

C2 - 17028778

AN - SCOPUS:33751002642

SN - 0141-5492

VL - 28

SP - 1965

EP - 1969

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 23

ER -

Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity

Abstract

Keywords

ASJC Scopus subject areas

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