Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity

Dong Hwan Lee, Jung Mo Kim, Seong Woo Kang, Ji Won Lee, Seung Wook Kim

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.

    Original languageEnglish
    Pages (from-to)1965-1969
    Number of pages5
    JournalBiotechnology letters
    Volume28
    Issue number23
    DOIs
    Publication statusPublished - 2006 Dec

    Bibliographical note

    Funding Information:
    Acknowledgment This work was supported by research grant 2003-N-BI03-P-01 from the Korea Energy Management Corporation.

    Keywords

    • Immobilization
    • Lipase
    • Pretreatment
    • Reuse
    • Soybean oil

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

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