Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea

Sung Hee Hong; Sang Eun Lee; Young Il Jeong; Heung Chul Kim; Sung Tae Chong; Terry A. Klein; Jin Won Song; Se Hun Gu; Shin Hyeong Cho; Won Ja Lee

doi:10.1016/j.vetpar.2014.07.032

Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea

Sung Hee Hong, Sang Eun Lee, Young Il Jeong, Heung Chul Kim, Sung Tae Chong, Terry A. Klein, Jin Won Song, Se Hun Gu, Shin Hyeong Cho, Won Ja Lee

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A survey was conducted to determine the distribution and prevalence of Toxoplasma gondii and Babesia microti infections in small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea (ROK). The serological prevalence of T. gondii (ELISA) and B. microti (IFAT) was 2.3% (15/667) and 2.1% (14/667), respectively. DNA extracts from small mammal heart tissues were screened by PCR for T. gondii and B. microti targeting regions of the GRA5 gene and the 18S rRNA and β-tubulin genes, respectively. Only 0.17% (1/578) of Apodemus agrarius was positive of T. gondii by PCR, while 0.52% (3/578) was positive of B. microti. All other small mammal species [Micromys minutus (16), Mus musculus (3), Myodes regulus (22), Microtus fortis (6), and Crocidura lasiura (42)] were negative for both T. gondii and B. microti. Based on sequence polymorphism and phylogenetic analysis, T. gondii closely aligned with Type I, a highly virulent strain, while B. microti positive samples closely aligned with US-type B. microti and others observed in the ROK, Russia, and Japan. These results indicate that A. agrarius is a reservoir for both T. gondii and B. microti in the ROK.

Original language	English
Pages (from-to)	512-517
Number of pages	6
Journal	Veterinary Parasitology
Volume	205
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetpar.2014.07.032
Publication status	Published - 2014

Bibliographical note

Funding Information:
Funding for this research was provided by the funding ( 4847-302-210-13 , 2011) from the Korea Centers for Disease Control and Prevention, Osong, ROK , the Armed Forces Health Surveillance Center , Global Emerging Infections Surveillance and Response System (AFHSC-GEIS), Silver Spring, MD , and the Public Health Command Region-Pacific, Camp Zama, Japan .

Publisher Copyright:
© 2014 Elsevier B.V.

Keywords

Apodemus agrarius
Babesia microti
Republic of Korea
Toxoplsama gondii

ASJC Scopus subject areas

Parasitology
General Veterinary

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10.1016/j.vetpar.2014.07.032

Cite this

Hong, S. H., Lee, S. E., Jeong, Y. I., Kim, H. C., Chong, S. T., Klein, T. A., Song, J. W., Gu, S. H., Cho, S. H., & Lee, W. J. (2014). Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea. Veterinary Parasitology, 205(3-4), 512-517. https://doi.org/10.1016/j.vetpar.2014.07.032

Hong, SH, Lee, SE, Jeong, YI, Kim, HC, Chong, ST, Klein, TA, Song, JW, Gu, SH, Cho, SH & Lee, WJ 2014, 'Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea', Veterinary Parasitology, vol. 205, no. 3-4, pp. 512-517. https://doi.org/10.1016/j.vetpar.2014.07.032

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title = "Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea",

abstract = "A survey was conducted to determine the distribution and prevalence of Toxoplasma gondii and Babesia microti infections in small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea (ROK). The serological prevalence of T. gondii (ELISA) and B. microti (IFAT) was 2.3% (15/667) and 2.1% (14/667), respectively. DNA extracts from small mammal heart tissues were screened by PCR for T. gondii and B. microti targeting regions of the GRA5 gene and the 18S rRNA and β-tubulin genes, respectively. Only 0.17% (1/578) of Apodemus agrarius was positive of T. gondii by PCR, while 0.52% (3/578) was positive of B. microti. All other small mammal species [Micromys minutus (16), Mus musculus (3), Myodes regulus (22), Microtus fortis (6), and Crocidura lasiura (42)] were negative for both T. gondii and B. microti. Based on sequence polymorphism and phylogenetic analysis, T. gondii closely aligned with Type I, a highly virulent strain, while B. microti positive samples closely aligned with US-type B. microti and others observed in the ROK, Russia, and Japan. These results indicate that A. agrarius is a reservoir for both T. gondii and B. microti in the ROK.",

keywords = "Apodemus agrarius, Babesia microti, Republic of Korea, Toxoplsama gondii",

author = "Hong, {Sung Hee} and Lee, {Sang Eun} and Jeong, {Young Il} and Kim, {Heung Chul} and Chong, {Sung Tae} and Klein, {Terry A.} and Song, {Jin Won} and Gu, {Se Hun} and Cho, {Shin Hyeong} and Lee, {Won Ja}",

note = "Funding Information: Funding for this research was provided by the funding ( 4847-302-210-13 , 2011) from the Korea Centers for Disease Control and Prevention, Osong, ROK , the Armed Forces Health Surveillance Center , Global Emerging Infections Surveillance and Response System (AFHSC-GEIS), Silver Spring, MD , and the Public Health Command Region-Pacific, Camp Zama, Japan . Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

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AU - Jeong, Young Il

AU - Kim, Heung Chul

AU - Chong, Sung Tae

AU - Klein, Terry A.

AU - Song, Jin Won

AU - Gu, Se Hun

AU - Cho, Shin Hyeong

AU - Lee, Won Ja

N1 - Funding Information: Funding for this research was provided by the funding ( 4847-302-210-13 , 2011) from the Korea Centers for Disease Control and Prevention, Osong, ROK , the Armed Forces Health Surveillance Center , Global Emerging Infections Surveillance and Response System (AFHSC-GEIS), Silver Spring, MD , and the Public Health Command Region-Pacific, Camp Zama, Japan . Publisher Copyright: © 2014 Elsevier B.V.

PY - 2014

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N2 - A survey was conducted to determine the distribution and prevalence of Toxoplasma gondii and Babesia microti infections in small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea (ROK). The serological prevalence of T. gondii (ELISA) and B. microti (IFAT) was 2.3% (15/667) and 2.1% (14/667), respectively. DNA extracts from small mammal heart tissues were screened by PCR for T. gondii and B. microti targeting regions of the GRA5 gene and the 18S rRNA and β-tubulin genes, respectively. Only 0.17% (1/578) of Apodemus agrarius was positive of T. gondii by PCR, while 0.52% (3/578) was positive of B. microti. All other small mammal species [Micromys minutus (16), Mus musculus (3), Myodes regulus (22), Microtus fortis (6), and Crocidura lasiura (42)] were negative for both T. gondii and B. microti. Based on sequence polymorphism and phylogenetic analysis, T. gondii closely aligned with Type I, a highly virulent strain, while B. microti positive samples closely aligned with US-type B. microti and others observed in the ROK, Russia, and Japan. These results indicate that A. agrarius is a reservoir for both T. gondii and B. microti in the ROK.

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Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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