PREVENTION OF EVAPORATION IN FEMTOLITER-SCALE WATER-IN-AIR DROPLET GENERATION FOR DIGITAL COUNTING OF BIOMOLECULES

Bo Hoon Han, Juhwan Park, Seok Chung, Ji Yoon Kang

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    The major challenge of digital counting using air-water interface droplet array is the prevention of evaporation. In this study, we generated the 100,000 droplets in a few seconds by passing the liquid plug to hydrophilic/hydrophobic array for digital counting of biomolecules. The evaporation of 30 fL-scale droplet array could be delayed from 2 seconds to more than 10 hours based on the simple clamping system and inversion of the device. Finally, digital enzyme linked immunosorbent assay (dELISA) was performed to verify the possibility of application of biochemical assay, and amyloid beta protein was quantified as a proof of concept.

    Original languageEnglish
    Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherChemical and Biological Microsystems Society
    Pages821-822
    Number of pages2
    ISBN (Electronic)9781733419031
    Publication statusPublished - 2021
    Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
    Duration: 2021 Oct 102021 Oct 14

    Publication series

    NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

    Conference

    Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
    Country/TerritoryUnited States
    CityPalm Springs, Virtual
    Period21/10/1021/10/14

    Bibliographical note

    Publisher Copyright:
    © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

    Keywords

    • Air-water interface droplet
    • Digital ELISA
    • Droplet array
    • Prevention of evaporation

    ASJC Scopus subject areas

    • Bioengineering
    • Chemical Engineering (miscellaneous)

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