Abstract
The major challenge of digital counting using air-water interface droplet array is the prevention of evaporation. In this study, we generated the 100,000 droplets in a few seconds by passing the liquid plug to hydrophilic/hydrophobic array for digital counting of biomolecules. The evaporation of 30 fL-scale droplet array could be delayed from 2 seconds to more than 10 hours based on the simple clamping system and inversion of the device. Finally, digital enzyme linked immunosorbent assay (dELISA) was performed to verify the possibility of application of biochemical assay, and amyloid beta protein was quantified as a proof of concept.
Original language | English |
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Title of host publication | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
Publisher | Chemical and Biological Microsystems Society |
Pages | 821-822 |
Number of pages | 2 |
ISBN (Electronic) | 9781733419031 |
Publication status | Published - 2021 |
Event | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 2021 Oct 10 → 2021 Oct 14 |
Publication series
Name | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
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Conference
Conference | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 |
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Country/Territory | United States |
City | Palm Springs, Virtual |
Period | 21/10/10 → 21/10/14 |
Bibliographical note
Publisher Copyright:© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
Keywords
- Air-water interface droplet
- Digital ELISA
- Droplet array
- Prevention of evaporation
ASJC Scopus subject areas
- Bioengineering
- Chemical Engineering (miscellaneous)