TY - JOUR
T1 - Probiotic potential of enterococcus faecium isolated from chicken cecum with immunomodulating activity and promoting longevity in caenorhabditis elegans
AU - Sim, Insuk
AU - Park, Keun Tae
AU - Kwon, Gayeung
AU - Koh, Jong Ho
AU - Lim, Young Hee
N1 - Funding Information:
This study was funded by the generous financial support of the Youlchon Foundation Nongshim Corporation and its affiliated companies.
Publisher Copyright:
© 2018 by The Korean Society for Microbiology and Biotechnology.
PY - 2018/6
Y1 - 2018/6
N2 - Probiotics, including Enterococcus faecium, confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faecium by 16S rDNA gene sequence analysis, and designated as E. faecium L11. To evaluate the potential of E. faecium L11 as a probiotic, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E. faecium L11 showed >66% and >62% survival in artificial gastric juice (0.3% pepsin, pH 2.5) and simulated small intestinal juice (0.5% bile salt and 0.1% pancreatin), respectively. Heat-killed E. faecium L11 significantly (p < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines (IL-6 and TNF-α) by activated macrophages obtained from ICR mice. In addition, E. faecium L11 showed a protective effect against Salmonella Typhimurium infection in Caenorhabditis elegans. In addition, feeding E. faecium L11 significantly (p < 0.05) extended the lifespan of C. elegans compared with the control. Furthermore, genes related to aging and host defense were upregulated in E. faecium L11-fed worms. In conclusion, E. faecium L11, which prolongs the lifespan of C. elegans, may be a potent probiotic supplement for livestock.
AB - Probiotics, including Enterococcus faecium, confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faecium by 16S rDNA gene sequence analysis, and designated as E. faecium L11. To evaluate the potential of E. faecium L11 as a probiotic, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E. faecium L11 showed >66% and >62% survival in artificial gastric juice (0.3% pepsin, pH 2.5) and simulated small intestinal juice (0.5% bile salt and 0.1% pancreatin), respectively. Heat-killed E. faecium L11 significantly (p < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines (IL-6 and TNF-α) by activated macrophages obtained from ICR mice. In addition, E. faecium L11 showed a protective effect against Salmonella Typhimurium infection in Caenorhabditis elegans. In addition, feeding E. faecium L11 significantly (p < 0.05) extended the lifespan of C. elegans compared with the control. Furthermore, genes related to aging and host defense were upregulated in E. faecium L11-fed worms. In conclusion, E. faecium L11, which prolongs the lifespan of C. elegans, may be a potent probiotic supplement for livestock.
KW - Caenorhabditis elegans
KW - Enterococcus faecium
KW - Immunomodulatory activity
KW - Lifespan extension
KW - Probiotics
UR - http://www.scopus.com/inward/record.url?scp=85049223263&partnerID=8YFLogxK
U2 - 10.4014/jmb.1802.02019
DO - 10.4014/jmb.1802.02019
M3 - Article
C2 - 29642285
AN - SCOPUS:85049223263
SN - 1017-7825
VL - 28
SP - 883
EP - 892
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 6
ER -