Probiotic potential of Staphylococcus hominis MBBL 2-9 as anti-Staphylococcus aureus agent isolated from the vaginal microbiota of a healthy woman

C. Sung, B. G. Kim, S. Kim, H. S. Joo, P. I. Kim

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    Aims: To isolate and characterize an antagonist for use as probiotic agent in the biocontrol of Staphylococcus aureus. Methods and Results: Bacteria that exhibited antimicrobial activity against Gram-positive bacteria including Staph. aureus were isolated from 12 healthy women, with Staphylococcus hominis MBBL 2-9 showing the strongest activity. The bacteriocin produced by Staph. hominis MBBL 2-9 was purified by 60% ammonium sulfate saturation, ultrafiltration, HLB cartridge and reverse-phase HPLC. The molecular weight was estimated as 2038·2 Da by MALDI-TOF mass spectrometry. The antagonist survived up to 2 h in artificial gastric juice (pH 2·5) and grew in the presence of 1% porcine bile extract. In addition, Staph. hominis MBBL 2-9 adhered effectively to HT-29 epithelial cell line. Conclusion: Staphylococcus hominis MBBL 2-9 exhibited desirable probiotic traits such as acid tolerance, bile resistance and adherence to epithelial cell line. The bacterium also produced a bacteriocin with unique molecular weight and high antimicrobial activity similar to traditional antibiotics. Significance and Impact of the Study: This study is the first report of a bacteriocin-producing Staph. hominis MBBL 2-9 that has potential for use as a probiotic agent against Staph. aureus.

    Original languageEnglish
    Pages (from-to)908-916
    Number of pages9
    JournalJournal of Applied Microbiology
    Volume108
    Issue number3
    DOIs
    Publication statusPublished - 2010 Mar

    Keywords

    • Antagonist
    • Bacteriocin
    • Biocontrol
    • Probiotics
    • Staphylococcus hominis MBBL 2-9

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

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