Abstract
One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.
Original language | English |
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Pages (from-to) | 1103-1111 |
Number of pages | 9 |
Journal | Applied Microbiology and Biotechnology |
Volume | 74 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2007 Apr |
Bibliographical note
Funding Information:Acknowledgment This study was supported by the Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.
Keywords
- Antagonistic effect
- Bifidobacterium thermacidophilum
- Clostridium perfringens
- Lactobacillus amylovorus
- Lactobacillus reuteri
- Lactobacillus salivarius
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology