Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract

Pyoung Il Kim, Min Young Jung, Young Hyo Chang, Saehun Kim, Seong Jae Kim, Yong Ha Park

    Research output: Contribution to journalArticlepeer-review

    87 Citations (Scopus)

    Abstract

    One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.

    Original languageEnglish
    Pages (from-to)1103-1111
    Number of pages9
    JournalApplied Microbiology and Biotechnology
    Volume74
    Issue number5
    DOIs
    Publication statusPublished - 2007 Apr

    Bibliographical note

    Funding Information:
    Acknowledgment This study was supported by the Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

    Keywords

    • Antagonistic effect
    • Bifidobacterium thermacidophilum
    • Clostridium perfringens
    • Lactobacillus amylovorus
    • Lactobacillus reuteri
    • Lactobacillus salivarius

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

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