TY - JOUR
T1 - Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract
AU - Kim, Pyoung Il
AU - Jung, Min Young
AU - Chang, Young Hyo
AU - Kim, Saehun
AU - Kim, Seong Jae
AU - Park, Yong Ha
N1 - Funding Information:
Acknowledgment This study was supported by the Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.
PY - 2007/4
Y1 - 2007/4
N2 - One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.
AB - One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. There was a large variation in the survival of the isolates in gastric juice and growth in the medium containing 0.3% (w/v) bile. L. salivarius G11 and L. amylovorus S6 adhered to the HT-29 epithelial cell line. Cell-free supernatant of L. amylovorus S6 showed higher antagonistic activity as effective as the antibiotics such as neomycin, chlortetracycline, and oxytetracycline against bacterial pathogens including C. perfringens, Salmonella typhimurium, Staphylococcus aureus, Vibrio cholerae, Edwardsiella tarda, and Aeromonas salmonicida subsp. salmonicida.
KW - Antagonistic effect
KW - Bifidobacterium thermacidophilum
KW - Clostridium perfringens
KW - Lactobacillus amylovorus
KW - Lactobacillus reuteri
KW - Lactobacillus salivarius
UR - http://www.scopus.com/inward/record.url?scp=33947271580&partnerID=8YFLogxK
U2 - 10.1007/s00253-006-0741-7
DO - 10.1007/s00253-006-0741-7
M3 - Article
C2 - 17136367
AN - SCOPUS:33947271580
SN - 0175-7598
VL - 74
SP - 1103
EP - 1111
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 5
ER -