Process strategy for 2,3-butanediol production in fed-batch culture by acetate addition

Sang Jun Lee, Han Suk Choi, Chan Kyum Kim, Laxmi Prasad Thapa, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Various strategies were studied to enhance 2,3-butanediol production using Enterobacter aerogenes SUMI014. The synergistic effect of acetate improved yield and productivity, and resulted in 32.3% increase in 2,3-butanediol production, compared to the wild strain. Optimizing the fermentation conditions successfully increased the 2,3-butanediol production. In batch fermentation, 93.75 g/L of 2,3-butanediol was obtained within 54 h, along with 0.49 g/g of yield and 1.74 g/L/h of productivity. The highest 2,3-butanediol production achieved was in fed-batch fermentation with acetate addition strategy, with production, yield and productivity of 126.10 g/L, 0.38 g/g and 2.10 g/L/h, respectively.

Original languageEnglish
Pages (from-to)157-162
Number of pages6
JournalJournal of Industrial and Engineering Chemistry
Publication statusPublished - 2017 Dec 25

Bibliographical note

Funding Information:
This study was supported by BK21 Plus program and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( NRF-2016R1D1A1A09918327 ).

Publisher Copyright:
© 2017 The Korean Society of Industrial and Engineering Chemistry


  • 2,3-Butanediol
  • Acetate supplementation
  • Enterobacter aerogenes
  • Fermentation
  • Lactate dehydrogenase

ASJC Scopus subject areas

  • Chemical Engineering(all)


Dive into the research topics of 'Process strategy for 2,3-butanediol production in fed-batch culture by acetate addition'. Together they form a unique fingerprint.

Cite this