TY - JOUR
T1 - Production and characterization of melanin pigments derived from Amorphotheca resinae
AU - Oh, Jeong Joo
AU - Kim, Jee Young
AU - Kwon, Sun Lul
AU - Hwang, Dong Hyeok
AU - Choi, Yoon E.
AU - Kim, Gyu Hyeok
N1 - Funding Information:
This research was supported by the Korea University Research Grant.
Publisher Copyright:
© 2020, The Microbiological Society of Korea.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - As melanin has emerged as functional pigment with cosmetic, health and food applications, the demand for the pigments is expected to increase. However, the conventional sources (e.g. mushroom, hair, and wool) of melanin production entail pigments inside the substrates which requires the costly extraction procedures, leading to inappropriate scalable production. In this study, we screened 102 of fungal isolates for their ability to produce melanin in the supernatant and selected the only Amorphotheca resinae as a promising candidate. In the peptone yeast extract glucose broth, A. resinae produced the melanin rapidly during the autolysis phase of growth, reaching up 4.5 g/L within 14 days. Structural characterization of the purified melanin from A. resinae was carried out by using elemental analysis, electron paramagnetic resonance, 13C solid-state nuclear magnetic resonance spectroscopy, and pyrolysis-gas chromatography-mass spectrometry in comparison with the standard melanins. The results indicate that the structural properties of A. resinae melanin is similar to the eumelanin which has a wide range of industrial uses. For example, the purified melanin from A. resinae has the potent antioxidant activities as a result of free radical scavenging assays. Consequently, A. resinae KUC3009 can be a promising candidate for scalable production of industrially applicable melanin.
AB - As melanin has emerged as functional pigment with cosmetic, health and food applications, the demand for the pigments is expected to increase. However, the conventional sources (e.g. mushroom, hair, and wool) of melanin production entail pigments inside the substrates which requires the costly extraction procedures, leading to inappropriate scalable production. In this study, we screened 102 of fungal isolates for their ability to produce melanin in the supernatant and selected the only Amorphotheca resinae as a promising candidate. In the peptone yeast extract glucose broth, A. resinae produced the melanin rapidly during the autolysis phase of growth, reaching up 4.5 g/L within 14 days. Structural characterization of the purified melanin from A. resinae was carried out by using elemental analysis, electron paramagnetic resonance, 13C solid-state nuclear magnetic resonance spectroscopy, and pyrolysis-gas chromatography-mass spectrometry in comparison with the standard melanins. The results indicate that the structural properties of A. resinae melanin is similar to the eumelanin which has a wide range of industrial uses. For example, the purified melanin from A. resinae has the potent antioxidant activities as a result of free radical scavenging assays. Consequently, A. resinae KUC3009 can be a promising candidate for scalable production of industrially applicable melanin.
KW - Amorphotheca resinae
KW - fungal melanins
KW - melanin pigments
UR - http://www.scopus.com/inward/record.url?scp=85085570882&partnerID=8YFLogxK
U2 - 10.1007/s12275-020-0054-z
DO - 10.1007/s12275-020-0054-z
M3 - Article
C2 - 32424578
AN - SCOPUS:85085570882
SN - 1225-8873
VL - 58
SP - 648
EP - 656
JO - Journal of Microbiology
JF - Journal of Microbiology
IS - 8
ER -