TY - JOUR
T1 - Production and secretion of indole alkaloids in hairy root cultures of Catharanthus roseus
T2 - Effects of in situ adsorption, fungal elicitation and permeabilization
AU - Sang Jun Sim, Jun Sim
AU - Ho Nam Chang, Nam Chang
AU - Jang Ryol Liu, Ryol Liu
AU - Kyung Hee Jung, Hee Jung
N1 - Funding Information:
This work was supported in part by the Korea Science and Engineering Foundation, and the BioProcess Engineering Research Center in Korea.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1994
Y1 - 1994
N2 - Various hairy root clones were derived by transforming two kinds of Cathanranthus roseus plants (Catharanthus roseus cvs. Little Linda and Little Delicata) with Agrobacterium rhizogenes ATCC 15834. Hairy root growth and indole alkaloid production were then investigated to select a high-yielding hairy root clone. Among three Amberlite resins (XAS-2, XAD-4, and ajmalicine from hairy root cultures, with an increase in total production. To enhance alkaloid production and secretion, a permeabilizing agent (dimethyl sulfoxide) and a fungal elicitor to provide physical and biochemical stress, respectively, together with in situ adsorption. Dimethyl sulfoxide (0.5% v/v) treatment with in situ adsorption using XAD-7 was found appropriate for releasing indole alkaloids from hairy roots without affecting cell viability. In addition, fungal elicitation by Penicillium sp. enhanced both alkaloid production and secretion. By combining in situ adsorption sequentially with these techniques, the release ratio of catharanthine and ajmalicine was enhanced up to 20 and 70%, respectively, which was 3.4 and 2 times higher than that obtained with in situ adsorption by XAD-7 alone. Over a 27-d culture period, the total amounts of catharanthine and ajmalicine produced were 67 and 30.15 mg/l, respectively. These results indicated that the in situ adsorption sequentially applied with permeabilization and fungal elicitation have a synergistic effect on the production and secretion of indole alkaloids.
AB - Various hairy root clones were derived by transforming two kinds of Cathanranthus roseus plants (Catharanthus roseus cvs. Little Linda and Little Delicata) with Agrobacterium rhizogenes ATCC 15834. Hairy root growth and indole alkaloid production were then investigated to select a high-yielding hairy root clone. Among three Amberlite resins (XAS-2, XAD-4, and ajmalicine from hairy root cultures, with an increase in total production. To enhance alkaloid production and secretion, a permeabilizing agent (dimethyl sulfoxide) and a fungal elicitor to provide physical and biochemical stress, respectively, together with in situ adsorption. Dimethyl sulfoxide (0.5% v/v) treatment with in situ adsorption using XAD-7 was found appropriate for releasing indole alkaloids from hairy roots without affecting cell viability. In addition, fungal elicitation by Penicillium sp. enhanced both alkaloid production and secretion. By combining in situ adsorption sequentially with these techniques, the release ratio of catharanthine and ajmalicine was enhanced up to 20 and 70%, respectively, which was 3.4 and 2 times higher than that obtained with in situ adsorption by XAD-7 alone. Over a 27-d culture period, the total amounts of catharanthine and ajmalicine produced were 67 and 30.15 mg/l, respectively. These results indicated that the in situ adsorption sequentially applied with permeabilization and fungal elicitation have a synergistic effect on the production and secretion of indole alkaloids.
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U2 - 10.1016/0922-338X(94)90295-X
DO - 10.1016/0922-338X(94)90295-X
M3 - Article
AN - SCOPUS:0027984628
SN - 1389-1723
VL - 78
SP - 229
EP - 234
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 3
ER -