Production, characterization, and epitope mapping of monoclonal antibodies of ribosomal protein S3 (rpS3)

Woo Sung Ahn, Tae Sung Kim, Yong Jun Park, Young Kwang Park, Hag Dong Kim, Joon Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Ribosomal protein S3 (rpS3), a member of 40S small ribosomal subunit, is a multifunctional protein with various extra-ribosomal functions including DNA repair endonuclease activity and is secreted from cancer cells. Therefore, antibodies with high specificity against rpS3 protein could be useful cancer biomarkers. In this study, polyclonal antibody (pAb) and monoclonal antibodies (mAbs) were raised against rpS3 protein and epitope mapping was performed for each antibody; the amino acid residues of rpS3 were scanned from amino acid 185 to 243 through peptide scanning to reveal the epitopes of each mAb. Results showed that pAb R2 has an epitope from amino acid 203 to 230, mAb M7 has an epitope from amino acid 213 to 221, and mAb M8 has an epitope from amino acid 197 to 219. Taken together, novel mAbs and pAb against rpS3 were raised and mapped against rpS3 with different specific epitopes.

Original languageEnglish
Pages (from-to)323-336
Number of pages14
JournalAnimal Cells and Systems
Volume25
Issue number5
DOIs
Publication statusPublished - 2021

Bibliographical note

Publisher Copyright:
© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Keywords

  • Ribosomal protein subunit small 3
  • conformational epitope
  • direct ELISA
  • epitope mapping
  • immunoprecipitation
  • linear form epitope
  • mAb
  • peptide synthesis
  • sandwich ELISA

ASJC Scopus subject areas

  • Animal Science and Zoology
  • General Biochemistry,Genetics and Molecular Biology

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