Abstract
Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. cerevisiae to produce 1,2-propanediol. Metabolically engineered S. cerevisiae strains with overexpression of glycerol dissimilation pathway genes, including glycerol kinase (GUT1), glycerol 3-phosphate dehydrogenase (GUT2), glycerol dehydrogenase (gdh), and a glycerol transporter gene (GUP1), showed increased glycerol utilization and growth rate. More significant improvement of glycerol utilization and growth rate was accomplished by introducing 1,2-propanediol pathway genes, mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase) from Escherichia coli. By engineering both glycerol dissimilation and 1,2-propanediol pathways, the glycerol utilization and growth rate were improved 141% and 77%, respectively, and a 2.19 g 1,2-propanediol/l titer was achieved in 1% (v/v) glycerolcontaining YEPD medium in engineered S. cerevisiae.
Original language | English |
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Pages (from-to) | 846-853 |
Number of pages | 8 |
Journal | Journal of microbiology and biotechnology |
Volume | 21 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2011 Aug |
Keywords
- 1,2-propanediol
- Glycerol
- Metabolic engineering
- Saccharomyces cerevisiae
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology