Production of 2′-fucosyllactose using ⍺1,2-fucosyltransferase from a GRAS bacterial strain

Jun Min Lee, Bu Soo Park, Min Kyu Oh

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

2′-Fucosyllactose (2′-FL) is a major oligosaccharide found in human breast milk. It is produced from GDP-L-fucose and D-lactose by ⍺1,2-fucosyltransferase (⍺1,2-fucT), but the enzyme has been identified mostly in pathogens. In this study, an ⍺1,2-fucT was isolated from a Generally Recognized as Safe (GRAS) Bacillus megaterium strain. The enzyme was successfully expressed in metabolically-engineered Escherichia coli. Furthermore, replacement of non-conserved amino acid residues with conserved ones in the protein led to an increase in the rate of 2′-FL production. As a result, fed-batch fermentation of E. coli produced 30 g/L of 2′-FL from glucose and lactose. Thus, the overproduction of 2′-FL using a novel enzyme from a GRAS bacteria strain was successfully demonstrated.

Original languageEnglish
Article number110232
JournalEnzyme and Microbial Technology
Volume167
DOIs
Publication statusPublished - 2023 Jun

Bibliographical note

Funding Information:
This research was supported by Korea University funding. The authors thank Bum-Seok Park and Jin-Young Kim for advice on experimental design and for transferring strain.

Publisher Copyright:
© 2023 Elsevier Inc.

Keywords

  • 2′-Fucosyllactose
  • Bacillus megaterium
  • GRAS
  • Human milk oligosaccharide (HMO)
  • ⍺1,2-fucosyltransferase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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