Background: 2'-Fucosyllactose (2-FL), one of the most abundant oligosaccharides in human milk, has potential applications in foods due to its health benefits such as the selective promotion of bifidobacterial growth and the inhibition of pathogenic microbial binding to the human gut. Owing to the limited amounts of 2-FL in human milk, alternative microbial production of 2-FL is considered promising. To date, microbial production of 2-FL has been studied mostly in Escherichia coli. In this study, 2-FL was produced alternatively by using a yeast Saccharomyces cerevisiae, which may have advantages over E. coli. Results: Fucose and lactose were used as the substrates for the salvage pathway which was constructed with fkp coding for a bifunctional enzyme exhibiting l-fucokinase and guanosine 5'-diphosphate-l-fucose phosphorylase activities, fucT2 coding for α-1,2-fucosyltransferase, and LAC12 coding for lactose permease. Production of 2-FL by the resulting engineered yeast was verified by mass spectrometry. 2-FL titers of 92 and 503mg/L were achieved from 48-h batch fermentation and 120-h fed-batch fermentation fed with ethanol as a carbon source, respectively. Conclusions: This is the first report on 2-FL production by using yeast S. cerevisiae. These results suggest that S. cerevisiae can be considered as a host engineered for producing 2-FLvia the salvage pathway.
Bibliographical noteFunding Information:
Facility support from the Institute of Biomedical Science and Food Safety at the Korea University Food Safety Hall is acknowledged. C1 Gas Refinery Program through the National Research Foundation of Korea (2016M3D3A1A01913268).
C1 Gas Refinery Program through the National Research Foundation of Korea (2016M3D3A1A01913268).
Facility support from the Institute of Biomedical Science and Food Safety at the Korea University Food Safety Hall is acknowledged.
© 2018 The Author(s).
- Human milk oligosaccharide
- Lactose permease
- Saccharomyces cerevisiae
- Salvage pathway
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology