Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35

Hyun Yong Shin, Jin Young Lee, Han Suk Choi, Ja Hyun Lee, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


In this study, cephalosporin C production by Acremonium chrysogenum M35 cultured with crude glycerol instead of rice oil and methionine was investigated. The addition of crude glycerol increased cephalosporin C production by 6-fold in shake-flask culture, and also the amount of cysteine. In fed-batch culture without methionine, crude glycerol resulted only in overall improvement in cephalosporin C production (about 700%). In addition, A. chrysogenum M35 became highly differentiated in fed-batch culture with crude glycerol, compared with the differentiation in batch culture. The results presented here suggest that crude glycerol can replace methionine and plant oil as cysteine and carbon sources during cephalosporin C production by A. chrysogenum M35.

Original languageEnglish
Pages (from-to)753-758
Number of pages6
JournalJournal of Microbiology
Issue number5
Publication statusPublished - 2011 Oct

Bibliographical note

Funding Information:
This work was supported by the Advenced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science and Technology (ABC-2010-0029799) and the core Environmental Technology Development project for Next Generation funded by the Ministry of Environment, Republic of Korea (No.032-091-019).


  • Acremonium chrysogenum M35
  • cephalosporin C
  • crude glycerol
  • cystathionine-γ-lyase activity
  • cysteine
  • methionine

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology


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