Abstract
Fucose is a rare sugar obtained from fucoidan in brown algae. However, fucoidan contains low amounts of fucose, making the process of obtaining fucose inefficient and expensive. Colanic acid (CA) is a capsular exopolysaccharide produced by Escherichia coli, which has great potential as a source of fucose owing to its high proportion of fucose. In this study, we aimed to efficiently obtain fucose from the hydrolysis of CA and then produce 2′-fucosyllactose (2′-FL) and 2′-fucosylgalactose (2′-FG) using fucose in the CA hydrolysate, using engineered yeast. For the acid hydrolysis of CA, optimal conditions were 110 °C, 4% (w/v) H3PO4, and 60 min. Using fucose from CA hydrolysate as a substrate, 2′-FL and 2′-FG were produced from engineered Saccharomyces cerevisiae. This is the first report of producing fucosylated oligosaccharides from CA. Considering the benefits of CA in obtaining fucose, these results can lead to further applications of CA as a fucose supplier.
| Original language | English |
|---|---|
| Pages (from-to) | 332-340 |
| Number of pages | 9 |
| Journal | Process Biochemistry |
| Volume | 122 |
| DOIs | |
| Publication status | Published - 2022 Nov |
Bibliographical note
Funding Information:This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries, funded by the Korea Ministry of Agriculture, Food, and Rural Affairs ( 32136-05-1-SB010 ) and the Mid-career Researcher Program through the National Research Foundation of Korea ( 2020R1A2B5B02002631 ). This work was also supported by the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety and by a Korea University Grant.
Publisher Copyright:
© 2022 Elsevier Ltd
Keywords
- 2′-Fucosylgalactose
- 2′-Fucosyllactose
- Acid hydrolysis
- Colanic acid
- Fucose
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology