Production of L-lactic acid from metabolically engineered strain of Enterobacter aerogenes ATCC 29007

Laxmi Prasad Thapa; Sang Jun Lee; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.enzmictec.2017.03.003

Production of L-lactic acid from metabolically engineered strain of Enterobacter aerogenes ATCC 29007

Laxmi Prasad Thapa, Sang Jun Lee, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

In this study, L-lactic acid production was investigated from metabolically engineered strain of E. aerogenes ATCC 29007. The engineered strain E. aerogenes SUMI01 (Δpta) was generated by the deletion of phosphate acetyltransferase (pta) gene from the chromosome of E. aerogenes ATCC 29007 and deletion was confirmed by colony PCR. Under the optimized fermentation conditions, at 37 °C and pH 6 for 84 h, the L-lactic acid produced by engineered strain E. aerogenes SUMI01 (Δpta) in flask fermentation using 100 g/L mannitol as the carbon source was 40.05 g/L as compared to that of the wild type counterpart 20.70 g/L. At the end of the batch fermentation in bioreactor the production of L-lactic acid reached to 46.02 g/L and yield was 0.41 g/g by utilizing 112.32 g/L mannitol. This is the first report regarding the production of L-lactic acid from Enterobacter species. We believe that this result may provide valuable guidelines for further engineering Enterobacter strain for the improvement of L-lactic acid production.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Enzyme and Microbial Technology
Volume	102
DOIs	https://doi.org/10.1016/j.enzmictec.2017.03.003
Publication status	Published - 2017 Jul 1

Bibliographical note

Funding Information:
This research was supported by the grant from the National Research Foundation of Korea (NRF), funded by the Korean Government (MSIP) (No. 2014R1A2A2A01007321) and the Industrial Strategic Technology Development Program (10051513) funded by the Ministry of Trade, Industry & Energy(MI, Korea).

Publisher Copyright:
© 2017 Elsevier Inc.

Keywords

E. aerogenes ATCC29007
E. aerogenes SUMI01
L-lactic acid
phosphate acetyltransferase

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.enzmictec.2017.03.003

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abstract = "In this study, L-lactic acid production was investigated from metabolically engineered strain of E. aerogenes ATCC 29007. The engineered strain E. aerogenes SUMI01 (Δpta) was generated by the deletion of phosphate acetyltransferase (pta) gene from the chromosome of E. aerogenes ATCC 29007 and deletion was confirmed by colony PCR. Under the optimized fermentation conditions, at 37 °C and pH 6 for 84 h, the L-lactic acid produced by engineered strain E. aerogenes SUMI01 (Δpta) in flask fermentation using 100 g/L mannitol as the carbon source was 40.05 g/L as compared to that of the wild type counterpart 20.70 g/L. At the end of the batch fermentation in bioreactor the production of L-lactic acid reached to 46.02 g/L and yield was 0.41 g/g by utilizing 112.32 g/L mannitol. This is the first report regarding the production of L-lactic acid from Enterobacter species. We believe that this result may provide valuable guidelines for further engineering Enterobacter strain for the improvement of L-lactic acid production.",

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note = "Funding Information: This research was supported by the grant from the National Research Foundation of Korea (NRF), funded by the Korean Government (MSIP) (No. 2014R1A2A2A01007321) and the Industrial Strategic Technology Development Program (10051513) funded by the Ministry of Trade, Industry & Energy(MI, Korea). Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

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N1 - Funding Information: This research was supported by the grant from the National Research Foundation of Korea (NRF), funded by the Korean Government (MSIP) (No. 2014R1A2A2A01007321) and the Industrial Strategic Technology Development Program (10051513) funded by the Ministry of Trade, Industry & Energy(MI, Korea). Publisher Copyright: © 2017 Elsevier Inc.

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N2 - In this study, L-lactic acid production was investigated from metabolically engineered strain of E. aerogenes ATCC 29007. The engineered strain E. aerogenes SUMI01 (Δpta) was generated by the deletion of phosphate acetyltransferase (pta) gene from the chromosome of E. aerogenes ATCC 29007 and deletion was confirmed by colony PCR. Under the optimized fermentation conditions, at 37 °C and pH 6 for 84 h, the L-lactic acid produced by engineered strain E. aerogenes SUMI01 (Δpta) in flask fermentation using 100 g/L mannitol as the carbon source was 40.05 g/L as compared to that of the wild type counterpart 20.70 g/L. At the end of the batch fermentation in bioreactor the production of L-lactic acid reached to 46.02 g/L and yield was 0.41 g/g by utilizing 112.32 g/L mannitol. This is the first report regarding the production of L-lactic acid from Enterobacter species. We believe that this result may provide valuable guidelines for further engineering Enterobacter strain for the improvement of L-lactic acid production.

AB - In this study, L-lactic acid production was investigated from metabolically engineered strain of E. aerogenes ATCC 29007. The engineered strain E. aerogenes SUMI01 (Δpta) was generated by the deletion of phosphate acetyltransferase (pta) gene from the chromosome of E. aerogenes ATCC 29007 and deletion was confirmed by colony PCR. Under the optimized fermentation conditions, at 37 °C and pH 6 for 84 h, the L-lactic acid produced by engineered strain E. aerogenes SUMI01 (Δpta) in flask fermentation using 100 g/L mannitol as the carbon source was 40.05 g/L as compared to that of the wild type counterpart 20.70 g/L. At the end of the batch fermentation in bioreactor the production of L-lactic acid reached to 46.02 g/L and yield was 0.41 g/g by utilizing 112.32 g/L mannitol. This is the first report regarding the production of L-lactic acid from Enterobacter species. We believe that this result may provide valuable guidelines for further engineering Enterobacter strain for the improvement of L-lactic acid production.

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Production of L-lactic acid from metabolically engineered strain of Enterobacter aerogenes ATCC 29007

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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