Production of S-methyl-methionine using engineered Saccharomyces cerevisiae sake K6

Jun Min Lee, Min Ho Park, Bu Soo Park, Min Kyu Oh

Research output: Contribution to journalArticlepeer-review


S-methyl-methionine (SMM), also known as vitamin U, is an important food supplement produced by various plants. In this study, we attempted to produce it in an engineered microorganism, Saccharomyces cerevisiae, by introducing an MMT gene encoding a methionine S-methyltransferase from Arabidopsis thaliana. The S. cerevisiae sake K6 strain, which is a Generally Recognized as Safe (GRAS) strain, was chosen as the host because it produces a significant amount of S-adenosylmethionine (SAM), a precursor of SMM. To increase SMM production in the host, MHT1 and SAM4 genes encoding homocysteine S-methyltransferase were knocked out to prevent SMM degradation. Additionally, MMP1, which encodes S-methyl-methionine permease, was deleted to prevent SMM from being imported into the cell. Finally, ACS2 gene encoding acetyl-CoA synthase was overexpressed, and MLS1 gene encoding malate synthase was deleted to increase SAM availability. Using the engineered strain, 1.92 g/L of SMM was produced by fed-batch fermentation. One-Sentence Summary: Introducing a plant-derived MMT gene encoding methionine S-methyltransferase into engineered Saccharomyces cerevisiae sake K6 allowed microbial production of S-methyl-methionine (SMM).

Original languageEnglish
Article numberkuad026
JournalJournal of Industrial Microbiology and Biotechnology
Issue number1
Publication statusPublished - 2023

Bibliographical note

Funding Information:
This work was supported by the Korea University funding.

Publisher Copyright:
© 2023 The Author(s) 2023.


  • Methioinine S-methyltransferase
  • S-adenosyl-methionine
  • S-methyl-methionine
  • Saccharomyces cerevisiae sake K6

ASJC Scopus subject areas

  • General Medicine


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